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Product Info Summary
| SKU: | M03417-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PEPD Mouse Monoclonal Antibody [Clone ID: OTI1B7]
SKU/Catalog Number
M03417-1
Size
100 µl
Description
Boster Bio Anti-PEPD mouse monoclonal antibody, clone OTI1B7 (formerly 1B7). Catalog# M03417-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PEPD Mouse Monoclonal Antibody [Clone ID: OTI1B7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03417-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1B7
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PEPD (NP_000276) produced in HEK293T cell.
Reactive Species
M03417-1 is reactive to PEPD in Human, Mouse, Rat
Observed Molecular Weight
55 kDa
Calculated molecular weight
54.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03417-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC: 1:150
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PEPD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PEPD.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PEPD monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PEPD monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PEPD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either PEPD (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PEPD antibody (M03417-1)
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Anti-PEPD mouse monoclonal antibody (M03417-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PEPD.
Specific Publications For Anti-PEPD Mouse Monoclonal Antibody [Clone ID: OTI1B7] (M03417-1)
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