Anti-PFKM/Pfk 1 Antibody

Boster Bio Anti-PFKM/Pfk 1 Antibody catalog # A04370. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04370
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-PFKM/Pfk 1 Antibody

See all Muscle Phosphofructokinase/PFKM/PFK-1 products

SKU/Catalog Number

A04370

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PFKM/Pfk 1 Antibody catalog # A04370. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PFKM/Pfk 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04370)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human PFKM.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04370 is reactive to PFKM in Human, Mouse, Rat

Applications

A04370 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PFKM (Source: Uniprot.Org, NCBI)

Uniprot ID

P08237

Gene ID

5213

Gene Name

PFKM

Full Name

ATP-dependent 6-phosphofructokinase, muscle type

Weight

85.183kDa

Superfamily

phosphofructokinase type A (PFKA) family

Alternative Names

6-phosphofructokinase, muscle type; EC 2.7.1,6-phosphofructo-1-kinase; EC 2.7.1.11; GSD7; MGC8699; Muscle Phosphofructokinase; PFK1; PFK-1; PFKA; PFK-A; PFKM; PFKX; Phosphofructo-1-kinase isozyme A; Phosphofructokinase 1; phosphofructokinase, muscle; phosphofructokinase, polypeptide X; Phosphofructokinase-1; Phosphofructokinase-M; Phosphohexokinase PFKM ATP-PFK, GSD7, PFK-1, PFK-A, PFK1, PFKA, PFKX, PPP1R122 phosphofructokinase, muscle ATP-dependent 6-phosphofructokinase, muscle type|6-phosphofructo-1-kinase|6-phosphofructokinase type A|6-phosphofructokinase, muscle type|phosphofructo-1-kinase isozyme A|phosphofructokinase 1|phosphofructokinase, polypeptide X|phosphofructokinase-M|phosphohexokinase|protein phosphatase 1, regulatory subunit 122

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on PFKM, check out the PFKM Infographic

PFKM infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for PFKM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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13 Customer Q&As for Anti-PFKM/Pfk 1 Antibody

Question

My question regarding product A04370, anti-PFKM/Pfk 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04370 anti-PFKM/Pfk 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-06

Question

Does anti-PFKM/Pfk 1 antibody A04370 work on bovine WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

Our lab technicians have not validated anti-PFKM/Pfk 1 antibody A04370 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PFKM/Pfk 1 antibody A04370 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-11

Question

I am looking for to test anti-PFKM/Pfk 1 antibody A04370 on rat thymus for research purposes, then I may be interested in using anti-PFKM/Pfk 1 antibody A04370 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

The products we sell, including anti-PFKM/Pfk 1 antibody A04370, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-08

Question

Do you have a BSA free version of anti-PFKM/Pfk 1 antibody A04370 available?

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PFKM/Pfk 1 antibody A04370 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-18

Question

Does anti-PFKM/Pfk 1 antibody A04370 work for WB with thymus?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

According to the expression profile of thymus, PFKM is highly expressed in thymus. So, it is likely that anti-PFKM/Pfk 1 antibody A04370 will work for WB with thymus.

Boster Scientific Support

Answered: 2019-07-25

Question

I see that the anti-PFKM/Pfk 1 antibody A04370 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-07-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-04

Question

Is a blocking peptide available for product anti-PFKM/Pfk 1 antibody (A04370)?

Verified Customer

Verified customer

Asked: 2018-12-10

Answer

We do provide the blocking peptide for product anti-PFKM/Pfk 1 antibody (A04370). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-12-10

Question

Here is the WB image, lot number and protocol we used for thymus using anti-PFKM/Pfk 1 antibody A04370. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-23

Question

Would A04370 anti-PFKM/Pfk 1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

It shows on the product datasheet, A04370 anti-PFKM/Pfk 1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-19

Question

Is this A04370 anti-PFKM/Pfk 1 antibody reactive to the isotypes of PFKM?

J. Wu

Verified customer

Asked: 2017-06-13

Answer

The immunogen of A04370 anti-PFKM/Pfk 1 antibody is Synthesized peptide derived from the Internal region of human PFKM. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-06-13

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-PFKM/Pfk 1 antibody A04370. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-05-24

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-05-24

Question

We are currently using anti-PFKM/Pfk 1 antibody A04370 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?

C. Edwards

Verified customer

Asked: 2016-08-24

Answer

The anti-PFKM/Pfk 1 antibody (A04370) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-24

Question

I was wanting to use your anti-PFKM/Pfk 1 antibody for WB for rat thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat thymus identification?

L. Zhang

Verified customer

Asked: 2013-11-25

Answer

It shows on the product datasheet, A04370 anti-PFKM/Pfk 1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat thymus in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-11-25

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A04370
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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