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Product Info Summary
| SKU: | A04370-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-PFKM Antibody
SKU/Catalog Number
A04370-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-PFKM Antibody catalog # A04370-1. Tested in WB,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFKM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04370-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human PFKM(NP_001160160.1).
Reactive Species
A04370-1 is reactive to PFKM in Human, Mouse, Rat
Calculated molecular weight
85.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04370-1 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using PFKM antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
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Immunofluorescence analysis of C6 cells using PFKM Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of L929 cells using PFKM Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using PFKM Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.
Specific Publications For Anti-PFKM Antibody (A04370-1)
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