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Product Info Summary
| SKU: | A12257 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PHKG1 Antibody
SKU/Catalog Number
A12257
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PHKG1 Antibody catalog # A12257. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PHKG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12257)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PHKG1. AA range:241-290
Reactive Species
A12257 is reactive to PHKG1 in Human, Mouse, Rat
Calculated molecular weight
45.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12257 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western Blot analysis of various cells using PHKG1 Polyclonal Antibody
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Western Blot analysis of HepG2 cells using PHKG1 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-PHKG1 Antibody (A12257)
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Customer Q&As
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4 Customer Q&As for Anti-PHKG1 Antibody
Question
Would A12257 anti-PHKG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
It shows on the product datasheet, A12257 anti-PHKG1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-02
Question
Is this A12257 anti-PHKG1 antibody reactive to the isotypes of PHKG1?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
The immunogen of A12257 anti-PHKG1 antibody is Synthesized peptide derived from the Internal region of human PHKG1. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-09
Question
We are currently using anti-PHKG1 antibody A12257 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
The anti-PHKG1 antibody (A12257) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-20
Question
We want to test anti-PHKG1 antibody A12257 on human muscle of leg for research purposes, then I may be interested in using anti-PHKG1 antibody A12257 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Evans
Verified customer
Asked: 2013-09-03
Answer
The products we sell, including anti-PHKG1 antibody A12257, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-09-03
