Anti-PHKG1 Antibody

PHKG1 antibody

Boster Bio Anti-PHKG1 Antibody catalog # A12257. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A12257
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-PHKG1 Antibody

View all PHKG1 Antibodies

SKU/Catalog Number

A12257

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PHKG1 Antibody catalog # A12257. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PHKG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12257)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

F7E4

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human PHKG1. at AA rangle: 210-290

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A12257 is reactive to PHKG1 in Human

Applications

A12257 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

45.024kDa

Background of PHKG1

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PHKG1 (Source: Uniprot.org, NCBI)

Gene Name

PHKG1

Full Name

Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform

Weight

45.024kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.19; PHKG; phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform; phosphorylase kinase gamma; Phosphorylase kinase subunit gamma-1; phosphorylase kinase, gamma 1 (muscle) PHKG1 PHKG phosphorylase kinase catalytic subunit gamma 1 phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform|PHK-gamma-M|phosphorylase kinase gamma subunit 1|phosphorylase kinase subunit gamma-1|phosphorylase kinase, gamma 1 (muscle)|serine/threonine-protein kinase PHKG1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PHKG1, check out the PHKG1 Infographic

PHKG1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PHKG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-PHKG1 Antibody

Question

Would A12257 anti-PHKG1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

It shows on the product datasheet, A12257 anti-PHKG1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-02

Question

Is this A12257 anti-PHKG1 antibody reactive to the isotypes of PHKG1?

Verified Customer

Verified customer

Asked: 2019-05-09

Answer

The immunogen of A12257 anti-PHKG1 antibody is Synthesized peptide derived from the Internal region of human PHKG1. at AA rangle: 210-290. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-09

Question

We are currently using anti-PHKG1 antibody A12257 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

The anti-PHKG1 antibody (A12257) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-12-20

Question

We want to test anti-PHKG1 antibody A12257 on human muscle of leg for research purposes, then I may be interested in using anti-PHKG1 antibody A12257 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

J. Evans

Verified customer

Asked: 2013-09-03

Answer

The products we sell, including anti-PHKG1 antibody A12257, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-09-03

Order DetailsPrice
A12257

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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