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Product Info Summary
| SKU: | A08432-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-LMTK3 Antibody Picoband®
SKU/Catalog Number
A08432-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-LMTK3 Antibody Picoband® catalog # A08432-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LMTK3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08432-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human LMTK3 recombinant protein (Position: S1317-D1382).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08432-2 is reactive to LMTK3 in Human, Mouse, Rat
Observed Molecular Weight
153 kDa
Calculated molecular weight
153.7 kDa
Background of LMTK3
Lemur tyrosine kinase 3 is a protein that in humans is encoded by the LMTK3 gene. It is mapped to 19q13.33. LMTK3 (lemur tyrosine kinase 3), also known as LMR3 or TYKLM3, is a 1,460 amino acid protein that contains one protein kinase domain. One of several members of the protein kinase superfamily, LMTK3 is expressed at low levels in brain and testis where it catalyzes the ATP-dependent phosphorylation of target proteins, thereby modifying their function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08432-2 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human HEK293 whole cell, human PC-3 whole cell, human U2OS whole cell, mouse thymus tissue,
IHC: mouse brain tissue, rat brain tissue
Validation Images & Assay Conditions
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Western blot analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates
Lane 2: human PC-3 whole cell lysates
Lane 3: human U2OS whole cell lysates
Lane 4: mouse thymus tissue lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMTK3 antigen affinity purified polyclonal antibody (Catalog # A08432-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LMTK3 at approximately 153KD. The expected band size for LMTK3 is at 153KD.
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IHC analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).
LMTK3 was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LMTK3 Antibody (A08432-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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IHC analysis of LMTK3 using anti-LMTK3 antibody (A08432-2).
LMTK3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-LMTK3 Antibody (A08432-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-LMTK3 Antibody Picoband® (A08432-2)
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1 Customer Q&As for Anti-LMTK3 Antibody Picoband®
Question
We are currently using anti-LMTK3 antibody A08432-2 for rat tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
J. Bhatt
Verified customer
Asked: 2015-08-17
Answer
The anti-LMTK3 antibody (A08432-2) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-17
