|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-TORC1 Antibody|
|Description||Rabbit IgG polyclonal antibody for CREB-regulated transcription coactivator 1(CRTC1) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-TORC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1987)|
|Replacement Item||This antibody may replace the following items: sc-271333|sc-271333-X|sc-365009|sc-365009-X|sc-365010|sc-365010-X|sc-390881|sc-390881-X|sc-46269|sc-46269-X|sc-46270|sc-46270-X|sc-67146|sc-67146-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human TORC1(506-521aa EQQMAARQANALSHQL), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||CREB-regulated transcription coactivator 1|
|Molecular Weight||67300 MW|
|Protein Function||Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, involved in late-phase long- term potentiation (L-LTP) maintenance at the Schaffer collateral- CA1 synapses. May be required for dendritic growth of developing cortical neurons (By similarity). In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. .|
|Tissue Specificity||Highly expressed in adult and fetal brain. Located to specific regions such as the prefrontal cortex and cerebellum. Very low expression in other tissues such as heart, spleen, lung, skeletal muscle, salivary gland, ovary and kidney. .|
|Sequence Similarities||Belongs to the TORC family.|
|Subcellular Localization||Cytoplasm. Nucleus. Cytoplasmic when phosphorylated by SIK or AMPK and when sequestered by 14-3-3 proteins (By similarity). Translocated to the nucleus on Ser-151 dephosphorylation, instigated by a number of factors including calcium ion and cAMP levels. Light stimulation triggers a nuclear accumulation in the suprachiasmatic nucleus (SCN) of the brain. .|
|Alternative Names||CREB-regulated transcription coactivator 1;Mucoepidermoid carcinoma translocated protein 1;Transducer of regulated cAMP response element-binding protein 1;TORC-1;Transducer of CREB protein 1;CRTC1 ;KIAA0616 , MECT1 , TORC1 , WAMTP1 ;|
|Research Areas|||signal transduction|second messenger|nucleotide messenger|camp| epigenetics and nuclear signaling|transcription|polymerase associated factors|pol ii transcription|polymerase|tafs|other factors| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial biogenesis||
Background for CREB-regulated transcription coactivator 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-TORC1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-TORC1 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human CRTC1, 29.5KD (162aa tag+ L460-Y568)
Lane 1: Recombinant Human CRTC1 Protein 10ng
Lane 2: Recombinant Human CRTC1 Protein 5ng
Lane 3: Recombinant Human CRTC1 Protein 2.5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,