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AKT1 antibody (P00024-2) performs well in IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 19 Publications

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p00024 2 akt1 primary antibodies ihc testing 2

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IHC analysis of AKT1 using anti-AKT1 antibody (P00024-2).
AKT1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-AKT1 Antibody (P00024-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody

Name: Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: P00024-2
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

AKT1 antibody

19 Publications|

1 Review

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IP, IF, IHC, ICC, WB

Tested Applications

AKT1 antibody was positively detected by WB in:

human Hela whole cell, human MCF-7 whole cell, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse NIH/3T3 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

AKT1 antibody was positively detected by IHC in:

human colorectal adenocarcinoma tissue, human prostate cancer tissue, human lung adenocarcinoma tissue, intestinal diffuse large B-cell lymphoma tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

AKT1 antibody was positively detected by ICC/IF in:

A549 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Product Information

Product Name

Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
View all AKT1 Antibodies

Form

Liquid

Uniprot

P31749

Gene ID

207

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

HEA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human AKT1

Observed Molecular Weight

65 kDa

Calculated Molecular Weight

55.7 kDa

Cite This Product

Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-2)

Protocols

Western Blot protocol for AKT1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for AKT1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for AKT1 antibody

Review Protocol

Publications

Synergistic Antitumor Activity and Neuroprotective Effects of FGF1/FGFR Inhibition with Oxaliplatin Chemotherapy

Published: 2026-02-10

Journal: Journal of Advanced Research

Application: WB

Impact Factor: 13.00

Species: Human

PMID: 41672244

SKU	P00024-2
Application	Western Blot
Sample	rat colon tissue
Sample Processing Description	RIPA lysis buffer supplemented with PMSF (100:1) was used to lyse the samples for 10 min. The lysates were centrifuged at 12,000 rpm for 15 min, and the supernatants were collected. Fivefold loading buffer was added, and the samples were denatured at 100 °C for 10 min before loading onto SDS-PAGE.
Other Reagents	Blocking buffer
Primary Antibody	Phospho-AKT1 (T450) Rabbit Monoclonal Antibody
Primary Incubation	1:1000, overnight at 4 ℃
Secondary Antibody	HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
Secondary Incubation	1:2000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows representative Western blot results of AKT1 (Phospho-T450) and the internal control Actin in rat colon tissues from different groups. Among the low, medium, and high doses of the traditional Chinese medicine, the high-dose group exhibits the best therapeutic effect. The target bands are clear and well defined, and the experimental results are satisfactory.

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Free Secondary Antibody

Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody

AKT1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Synergistic Antitumor Activity and Neuroprotective Effects of FGF1/FGFR Inhibition with Oxaliplatin Chemotherapy

Repurposing Lamivudine: A novel therapeutic strategy for ischemic stroke targeting the glycolytic enzyme Pgk1

Smart biodegradable hydrogels incorporating a photoactive Ir(III) complex for NIR-triggered oral cancer treatment

AMPs inhibit the proliferation, migration, and invasion of lung cancer via the CHRM3/PI3K/AKT and CHRM3/MAPK pathways

Phytochemical Composition and Skin-Friendly Activities of the Ethyl Acetate Fraction in Ophioglossum vulgatum Linn., an In Vitro Study

Upregulation of PD-L1 contributes to improving the apoptosis of granulosa cells via the PI3K/AKT pathway in PCOS

Pungent agents derived from the fruits of Zanthoxylum armatum DC. are beneficial for ameliorating type 2 diabetes mellitus via regulation of AMPK/PI3K/Akt signaling

Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro

Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice

Sulfathiazole treats type 2 diabetes by restoring metabolism through activating CYP19A1

Resveratrol inhibits paclitaxel-induced neuropathic pain by the activation of PI3K/Akt and SIRT1/PGC1α pathway

Primaquine activates Keratin 7 to treat diabetes and its complications

Integration of pharmacodynamics and metabolomics reveals the therapeutic effects of 6-acetylacteoside on ovariectomy-induced osteoporosis mice

Terazosin Stimulates Pgk1 to Remedy Gastrointestinal Disorders

Cichorium pumilum Jacq Extract Inhibits LPS-Induced Inflammation via MAPK Signaling Pathway and Protects Rats From Hepatic Fibrosis Caused by Abnormalities in the Gut-Liver Axis

Identification and profiling of microRNAs expressed in oral buccal mucosa squamous cell carcinoma of Chinese hamster

Polyamine analogue QMA attenuated ischemic injury in MCAO rats via ERK and Akt activated Nrf2/HO-1 signaling pathway

Perfluorooctanoic acid impaired glucose homeostasis through affecting adipose AKT pathway

Protective Effects of Sodium (±)-5-Bromo-2-(α-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia

Review

Have you used Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody?

1 Review For Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody

This antibody is highly specific and efficient, suitable for Western blot detection of AKT1 (Phospho-T450) protein in rat colon, with only minor nonspecific bands observed.

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