Product Info Summary
SKU: | P00014-1 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00014-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody catalog # P00014-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00014-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEE-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-ATM (S1981)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00014-1 is reactive to ATM in Human
Applications
P00014-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
185 kDa
Calculated molecular weight
350.687kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-ATM (Ser1981) in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with Doxorubicin.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human liver, using Phospho-ATM (S1981) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For ATM (Source: Uniprot.org, NCBI)
Gene Name
ATM
Full Name
Serine-protein kinase ATM
Weight
350.687kDa
Superfamily
PI3/PI4-kinase family
Alternative Names
AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL ATM AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1 ATM serine/threonine kinase serine-protein kinase ATM|A-T mutated|AT mutated|TEL1, telomere maintenance 1, homolog|ataxia telangiectasia mutated
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATM, check out the ATM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody (P00014-1)
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No publications found for P00014-1
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-ATM (S1981) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-ATM (S1981) Rabbit Monoclonal antibody P00014-1 for human tissue, and we are satisfied with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-Phospho-ATM (S1981) Rabbit Monoclonal antibody (P00014-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13
Question
We ordered your anti-Phospho-ATM (S1981) Rabbit Monoclonal antibody for IF on liver in the past. I am using human, and We want to use the antibody for ICC next. I would like examining liver as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
I looked at the website and datasheets of our anti-Phospho-ATM (S1981) Rabbit Monoclonal antibody and it seems that P00014-1 has been tested on human in both IF and ICC. Thus P00014-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-12
Question
We have observed staining in human cervix carcinoma. Are there any suggestions? Is anti-Phospho-ATM (S1981) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
From what I have seen in literature cervix carcinoma does express ATM. From what I have seen in Uniprot.org, ATM is expressed in corpus callosum, fibroblast, brain, embryonic kidney, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-08-08
Question
Our lab were happy with the WB result of your anti-Phospho-ATM (S1981) Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma nucleus using this antibody. Is that expected? Could you tell me where is ATM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
According to literature, cervix carcinoma does express ATM. Generally ATM expresses in nucleus. Regarding which tissues have ATM expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Fibroblast, Pubmed ID: 7792600
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-06-10