Anti-DNA repair protein XRCC3 XRCC3 Antibody
Rabbit IgG polyclonal antibody for DNA repair protein XRCC3(XRCC3) detection. Tested with WB, IHC-P in Mouse;Rat.
Anti-DNA repair protein XRCC3 XRCC3 Antibody Info At A Glance
|Product Name||Anti-DNA repair protein XRCC3 XRCC3 Antibody
See all Xrcc3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for XRCC3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. XRCC3 information: Molecular Weight: 38446 MW; Subcellular Localization: Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Mitochondrion matrix . Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.|
|Cite This Product||Anti-DNA repair protein XRCC3 XRCC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1697)|
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|Anti-DNA repair protein XRCC3 XRCC3 Antibody may replace the following items: sc 5904|sc 11430|sc 5905|sc 271714|sc 53471.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse XRCC3(290-305aa NQLLMRLMVDRTHEDD), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Xrcc3 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||74335|
|Species Of This Entry||Mouse|
|Protein Name||DNA repair protein XRCC3|
|Alternative Names||Xrcc3|CMM6; DNA repair protein XRCC3; X-ray repair complementing defective repair in Chinese hamster cells 3; X-ray repair cross-complementing protein 3|
|Post Tranlational Modifications||Phosphorylation; Methylation; Acetylation; Oxidation|
|Gene Location||12|12 F1, on Chromasome 12, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Xrcc3 (Source: Uniprot.org, NCBI)
|Protein Function||Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2- dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C (By similarity). .|
|Research Areas||Apoptosis; Cancer; DNA Repair; Genes Sensitive to DNA Damaging Agents; Homologous Recombination; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Malignant Neoplasm Of Lung; Carcinoma; Lung Neoplasms; Malignant Squamous Cell Neoplasm; Cytogenetic Abnormality; Adenocarcinoma; Malignant Neoplasm Of Urinary Bladder; Colorectal Neoplasms; Carcinogenesis; Non-small Cell Lung Carcinoma; Bladder Neoplasm; Colorectal Cancer; Xeroderma; Xeroderma Pigmentosum; Head And Neck Neoplasms; Malignant Head And Neck Neoplasm|
|Related Pathways||Dna Repair; Double-strand Break Repair; Cell Cycle; Recombinational Repair; Mismatch Repair; Pathogenesis; Hypersensitivity; Dna Replication; Meiosis; Gene Conversion; S Phase; Localization; Mitosis; Cell Proliferation; Telomere Maintenance; Single Strand Break Repair; Dna Recombination; Chromosome Segregation; Sensitization; Menarche|
|Background||XRCC3(X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 3) is a DNA repair protein that in humans, is encoded by the XRCC3 gene. The XRCC3 is a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. XRCC3 interacts directly with RAD51 and may cooperate with RAD51 during recombinational repair. XRCC3 function is not limited to HR initiation, but extends to later stages in formation and resolution of HR intermediates, possibly by stabilizing heteroduplex DNA. The gene functionally complements Chinese hamster irs1SF, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents and is chromosomally unstable. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.|
|Scientific References||PMID: 16141072 by Carninci P., et al. The transcriptional landscape of the mammalian genome.|
Our Boster Quality Guarantee for Anti-DNA repair protein XRCC3 XRCC3 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse Brain Tissue Lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti--XRCC3 antigen affinity purified polyclonal antibody (Catalog # PA1697) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for -XRCC3 at approximately 38KD. The expected band size for -XRCC3 is at 38KD.
XRCC3 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- XRCC3 Antibody (PA1697) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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