Product Info Summary
SKU: | A00004S37 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-Catenin-beta (S37) CTNNB1 Antibody
View all beta-Catenin Antibodies
SKU/Catalog Number
A00004S37
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-Catenin-beta (S37) CTNNB1 Antibody catalog # A00004S37. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-Catenin-beta (S37) CTNNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00004S37)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
PI9-17
Isotype
IgG
Immunogen
Synthesized peptide derived from human Catenin-beta around the phosphorylation site of S37.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Weakly cross-reacts with dog p53.
Reactive Species
A00004S37 is reactive to CTNNB1 in Human, Mouse, Rat
Applications
A00004S37 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
85.497kDa
Background of beta-Catenin
Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-kidney tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Catenin-Beta (phospho Ser37) Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Western blot (WB) analysis of Catenin-beta (phospho Ser37) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Western blot (WB) analysis of Catenin-beta (phospho Ser37) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CTNNB1 (Source: Uniprot.org, NCBI)
Gene Name
CTNNB1
Full Name
Catenin beta-1
Weight
85.497kDa
Superfamily
beta-catenin family
Alternative Names
bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923 CTNNB1 CTNNB, EVR7, MRD19, NEDSDV, armadillo catenin beta 1 catenin beta-1|catenin (cadherin-associated protein), beta 1, 88kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTNNB1, check out the CTNNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTNNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Catenin-beta (S37) CTNNB1 Antibody (A00004S37)
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No publications found for A00004S37
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