Anti-beta Catenin/CTNNB1 Antibody
Rabbit IgG polyclonal antibody for Catenin beta-1(CTNNB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat. Cited in 19 publication(s).
Anti-beta Catenin/CTNNB1 Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
|Product Name||Anti-beta Catenin/CTNNB1 Antibody
See all beta-Catenin primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for BETA CATENIN/CTNNB1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. BETA CATENIN/CTNNB1 information: Molecular Weight: 85497 MW; Subcellular Localization: Cytoplasm. Nucleus . Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells; Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level).|
|Cite This Product||Anti-beta Catenin/CTNNB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1212)|
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|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human beta Catenin(764-781aa DGLPPGDSNQLAWFDTDL), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CTNNB1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||1499|
|Species Of This Entry||Human|
|Protein Name||Catenin beta-1|
|Alternative Names||beta-Catenin|bCatenin; beta 1 (88kD); beta-Catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta-1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923|
|Post Tranlational Modifications||Phosphorylation; Methylation; Cleavage; Ubiquitination; Dephosphorylation; Acetylation; Demethylation; Reduction; Glycosylation; Biotinylation; Deacetylation; Sumoylation; Amidation; Phosphorothioation; Isgylation; Prenylation; Acylation; Ribosylation; Nitration; Farnesylation|
|Subcellular Localization||Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Cell junction, adherens junction. Cell junction. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cell junction, synapse. Cytoplasm, cytoskeleton, cilium basal body. Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction|
|Gene Location||3p22.1, on Chromasome 3, gene sequence: NC_000003.12 (41199422..41240445)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CTNNB1 (Source: Uniprot.org, NCBI)
|Protein Function||Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E- cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).|
|Tissue Specificity||Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. Present in cortical neurons (at protein level). Expressed in breast cancer tissues (at protein level) (PubMed:29367600).|
|Related Diseases||Neoplasms; Malignant Neoplasms; Carcinoma; Tissue Adhesions; Cell Transformation, Neoplastic; Adenomatous Polyposis Coli; Polyposis; Neoplasm Metastasis; Colorectal Cancer; Colorectal Neoplasms; Adenocarcinoma; Carcinogenesis; Malignant Tumor Of Colon; Malignant Paraganglionic Neoplasm; Cell Invasion; Colonic Neoplasms; Adenoma; Neoplasm Invasiveness; Liver Neoplasms; Liver Carcinoma|
|Related Pathways||Localization; Cell Adhesion; Cell-cell Adhesion; Cell Proliferation; Pathogenesis; Cell Growth; Cell Cycle; Methylation; Cell Migration; Cell Death; Angiogenesis; Oncogenesis; Cell Motility; Cell Differentiation; Rna Interference; Mismatch Repair; Cell Cycle Arrest; Proteolysis; Dedifferentiation; Secretion|
|Background||Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development(both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.|
|Scientific References||PMID: 7806582 by Huelsken J., et al. E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton.|
PMID: 12019147 by Kim J.-S., et al. Oncogenic beta-catenin is required for bone morphogenetic protein 4 expression in human cancer cells.
Our Boster Quality Guarantee for Anti-beta Catenin/CTNNB1 Antibody covers its use in the following applications.Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: MM453 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: HELA Cell Lysate
IHC(P): Human Mammary Cancer Tissue
IHC(F): Rat Intestine Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 19
|Zhao P, Li Y, Gao G, Wang S, Yan Y, Zhan X, Liu Z, Mao Z, Chen S, Wang L. Eur J Med Chem. 2014 Oct 30;86:165-74. Doi: 10.1016/J.Ejmech.2014.08.049. Epub 2014 Aug 15. Design, Synthesis And Biological Evaluation Of N-Alkyl Or Aryl Substituted Isoind...||PubMed ID 25151579|
|Hao Hp, Wen Lb, Li Jr, Wang Y, Ni B, Wang R, Wang X, Sun Mx, Fan Hj, Mao X. Antiviral Res. 2015 May;117:99-109. Doi: 10.1016/J.Antiviral.2015.02.010. Epub 2015 Mar 5. Licl Inhibits Prrsv Infection By Enhancing Wnt/??-Catenin Pathway And Suppressin...||PubMed ID 25746333|
|Lyu Z, Wang H, Wang Y, Ding K, Liu H, Yuan L, Shi X, Wang M, Wang Y, Chen H. Nanoscale. 2014 Jun 21;6(12):6959-69. Doi: 10.1039/C4Nr01540A. Maintaining The Pluripotency Of Mouse Embryonic Stem Cells On Gold Nanoparticle Layers With Nanoscale But N...||PubMed ID 24839204|
|Zhang JH, Jiao LY, Li TJ, Zhu YY, Zhou JW, Tian J. J Cancer. 2017 Jun 3;8(9):1598-1608. doi: 10.7150/jca.18744. eCollection 2017. GSK-3β suppresses HCC cell dissociation in vitro by upregulating epithelial junction proteins and inhibiting Wnt/β-ca...||PubMed ID 28775779|
|Li C, Liu J, Zhang Q, Cui K, Ge Q, Wang C, Chen Z. Int J Oncol. 2018 Jun;52(6):1815-1826. doi: 10.3892/ijo.2018.4346. Epub 2018 Mar 29. Upregulation of E-cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder...||PubMed ID 29620261|
|Guo F, Yi X, Li M, Fu J, Li S. Exp Ther Med. 2017 Nov;14(5):4231-4237. doi: 10.3892/etm.2017.5084. Epub 2017 Aug 31. Snail1 is positively correlated with atrial fibrosis in patients with atrial fibrillation and rheumatic heart disease||PubMed ID 29104639|
|Modulating hair follicle size with?Wnt10b-DKK1?pair during hair regeneration||PubMed ID 24750467|
|Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells||PubMed ID 25286828|
|Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition||PubMed ID 26793111|
|Naringin ameliorates bone loss induced by sciatic neurectomy and increases Semaphorin 3A expression in denervated bone||PubMed ID 27109829|
|Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway||PubMed ID 25995040|
|Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway||PubMed ID 26677102|
|Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells||PubMed ID 26722422|
|Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis||PubMed ID 27012825|
|Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/?-catenin pathway activity||PubMed ID 27279629|
|MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway||PubMed ID 29201165|
|Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells||PubMed ID 29250149|
|Anti-cancer drug 3, 3%u2032-diindolylmethane activates Wnt4 signaling to enhance gastric cancer cell stemness and tumorigenesis||PubMed ID 26918831|
|Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat||PubMed ID 18681964|
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