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- Table of Contents
CTNNB1 antibody (PA1212) performs well in IP, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 16 Publications
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IHC analysis of CTNNB1 using anti-CTNNB1 antibody (PA1212).
CTNNB1 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CTNNB1 Antibody (PA1212) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CTNNB1 was detected in a paraffin-embedded section of human endometrial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CTNNB1 Antibody (PA1212) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-beta Catenin/CTNNB1 Antibody Picoband®
CTNNB1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IP, IHC, WB |
Tested Applications
CTNNB1 antibody was positively detected by WB in:
human HepG2 whole cell, human HeLa whole cell, human MCF-7 whole cell, human A431 whole cell, human RT4 whole cell, human SiHa whole cell, human T47D whole cell, human THP-1 whole cell, rat brain tissue, rat liver tissue, rat C6 whole cell, mouse brain tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CTNNB1 antibody was positively detected by IHC in:
human endometrial carcinoma tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CTNNB1 antibody was positively detected by IP in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-beta Catenin/CTNNB1 Antibody Picoband®
View all CTNNB1 Antibodies
View all CTNNB1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human beta Catenin, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
85.5 kDa
Background of CTNNB1
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Cite This Product
Anti-beta Catenin/CTNNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1212)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
100%
High Homology
Rabbit
94%
High Homology
Drosophila
61%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CTNNB1 antibody
Immunohistochemistry (IHC) protocol for CTNNB1 antibody
Immunoprecipitation (IP) protocol for CTNNB1 antibody
Publications
Application:
Species:
Decitabine Inhibits the Malignant Transformation of Oral Potentially Malignant Disorders by Upregulating SOX1 Expression via Demethylation to Modulate the Wnt Pathway in a Male Rat Model
Published: 2026-05-16
Journal: ARCHIVES OF ORAL BIOLOGY
Application: WB
Impact Factor: 2.30
Species: Human
PMID: 42166845
STEAP1 Suppresses Oral Squamous Cell Carcinoma by Targeting Wnt/β‐Catenin Signalling and EMT
Published: 2026-04-27
Journal: Cancer Medicine
Application: WB
Impact Factor: 3.10
Species: Human
PMID: 42046243
Saracatinib Delays the Progression of Renal Interstitial Fibrosis by Inhibiting the Wnt/β-Catenin Pathway
Published: 2025-09-22
Journal: Kidney360
Application: IHC,WB
Impact Factor: 3.00
Species: Human,Rat
PMID: 40982336
Effects of solamargine in hepatic metastasis of colorectal cancer: induction of ferroptosis and elimination of cancer stem cells
Published: 2025-07-11
Journal: Chinese Medicine
Application: WB,IHC
Impact Factor: 5.70
Species: Mouse,Human
PMID: 40646619
AHR regulates liver enlargement and regeneration through the YAP signaling pathway
Published: 2024-09-02
Journal: Heliyon
Application: IHC
Impact Factor: 3.40
Species: Mouse
PMID: 39296106
Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer
Published: 2024-05-28
Journal: Scientific Reports
Application: WB
Impact Factor: 3.80
Species: Human
PMID: 38806610
VEGF overexpression in transplanted NSCs promote recovery of neurological function in rats with cerebral ischemia by modulating the Wnt signal transduction pathway
Published: 2024-02-07
Journal: NEUROSCIENCE LETTERS
Application: IHC
Impact Factor: 2.50
Species: Rat
PMID: 38331020
LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer
Published: 2021-02-22
Journal: Journal of Cancer
Application: WB
Impact Factor: 4.21
Species: Human
PMID: 33758610
Effects of Wnt10b on dermal papilla cells via the canonical Wnt/β-catenin signalling pathway in the Angora rabbit
Published: 2019-06-26
Journal: JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
Application: WB,IF
Impact Factor: 1.70
Species: Rabbit
PMID: 31241227
Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway
Published: 2018-12-11
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: WB
Impact Factor: 5.50
Species: Rat,Human
PMID: 30562742
Retracted: Curcumin derivative L6H4 inhibits proliferation and invasion of gastric cancer cell line BGC-823
Published: 2018-09-22
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: IHC
Impact Factor: 2.96
Species: Mouse
PMID: 30242876
Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cells
Published: 2018-09-18
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 3.33
Species: Human
PMID: 30272277
Effect of FH535 on in vitro maturation of porcine oocytes by inhibiting WNT signaling pathway
Published: 2017-12-28
Journal: ANIMAL SCIENCE JOURNAL
Application: WB
Impact Factor: 1.33
Species: Pig
PMID: 29284185
Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody-antigen interaction and increased specificity
Published: 2017-07-13
Journal: ELECTROPHORESIS
Application: WB
Impact Factor: 2.74
Species: Rat,Mouse
PMID: 28704589
Stimulating Effect of a Newly Synthesized Sulfonamido-Basedgallate on Articular Chondrocytes in Vitro
Published: 2015-09-30
Journal: CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
Application: IHC
Impact Factor: 2.88
Species: Rat
PMID: 26418252
LiCl inhibits PRRSV infection by enhancing Wnt/β-catenin pathway and suppressing inflammatory responses
Published: 2015-03-04
Journal: ANTIVIRAL RESEARCH
Application: WB
Impact Factor: 3.94
Species: Pig
PMID: 25746333
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Product Promise
We promise Anti-beta Catenin/CTNNB1 Antibody Picoband® performs excellently in relevant applications for IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1212
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 12, 2026