Anti-beta Catenin/CTNNB1 Antibody Picoband®

Anti-beta Catenin antibody, PA1212, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: HELA Cell Lysate

Anti-beta Catenin antibody, PA1212, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Anti-beta Catenin antibody, PA1212, IHC(F)
IHC(F): Rat Intestine Tissue

TOP2A is involved in the progression and development of NSCLC via the Wnt/β-catenin signaling cascade. ( A ) TCGA enrichment analysis revealed that TOP2A expression is enriched in the Wnt signaling pathway (n = 1037). ( B ) Several NSCLC cell lines exhibited a clear association between the transcription of TOP2A and β-catenin. ( C ) The online GEPIA exhibited a correlation between TOP2A and WNT3A gene expression levels. ( D,E ) TOP2A overexpression and knockdown groups were examined for gene and protein levels of TOP2A, Wnt3a, and β-catenin. ( F ) Immunohistochemical assessment of Wnt3a and β-catenin expression in the TOP2A-positive and TOP2A-negative groups (200×). ( G ) Patients with high Wnt3a overexpression had an abysmal survival rate. *P < 0.05, **P < 0.01, or ***P < 0.001. All experiments were repeated three times independently.
Index in PubMed under a CC BY license. PMID: 38806610

TOP2A promotes non-small cell lung cancer progression via Wnt/β-catenin signaling pathway. ( A ) After Wnt3a gene knockdown, the mobility and invading capabilities of TOP2A-overexpressing cells were evaluated using the transwell assay. ( B,C ) Expression of components of Wnt/β-catenin signaling pathway and EMT-related proteins was examined using western blotting. ( D ) Flow cytometry was utilized to examine the cell cycle. ( E ) Analysis of apoptosis via flow cytometry. *P < 0.05, **P < 0.01, ***P < 0.001 or ****P < 0.0001; ns: not signiffcant.
Index in PubMed under a CC BY license. PMID: 38806610

SM impedes traits of CSCs partially through suppressing β-catenin in CRC. A Western blotting, B ALDH + staining and C tumorsphere formation assay were performed after CRC cells transduced with lentiviral β-catenin (encoded by the CTNNB1 gene) cDNA-expressing construct in the presence or absence of SM. Scale bar: 100 μm. D Western blotting, E ALDH + staining and F tumorsphere formation assays were conducted after CRC cells stably transduced with lentiviral shRNA against β-catenin with or without SM treatment. Scale bar: 100 μm. The values are represented as mean ± SD. * p < 0.05, *** p < 0.001
Index in PubMed under a CC BY license. PMID: 40646619

SM inhibits hepatic metastasis of CRC cells partially by attenuating β-catenin. A Transwell migration and B matrigel invasion assays were conducted followed by CRC cells transduced with lentiviral β-catenin-encoding construct with or without SM treatment. Scale bar: 100 μm. C Transwell migration and D matrigel invasion assays were conducted followed by CRC cells transduced with lentiviral shRNA against β-catenin in the presence or absence of SM. Scale bar: 100 μm. E The overexpression efficiency of β-catenin in MC38-Luc cells was examined by Western blotting analysis. F Representative images and G quantitative analysis of photon flux on day 21 of C57BL/6 mice followed by intrasplenic injection of MC38-Luc cells stably express β-catenin. n = 5 per group. H Representative images and quantitative analysis of liver metastasis in C57BL/6 mice (n = 5) measured by bioluminescence imaging. I Representative images of metastatic livers and ( J ) the number of surface foci in the livers in C57BL/6 mice (n = 5) are shown. Arrows indicate the presence of metastatic nodules. K Representative photographs of H&E staining of the liver section and L the number of liver metastatic foci in microscopic fields are presented (n = 3). Scale bar: 200 μm. The values are represented as mean ± SD. * p < 0.05, *** p < 0.001
Index in PubMed under a CC BY license. PMID: 40646619

IF analysis of Beta Catenin using anti-Beta Catenin antibody (PA1212).
Beta Catenin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Beta Catenin Antibody (PA1212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.