Anti-Phospho-CREB-1 (S142) Antibody

Product Name

Anti-Phospho-CREB-1 (S142) Antibody

View all CREB Antibodies

SKU/Catalog Number

A00577S142-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-CREB-1 (S142) Antibody catalog # A00577S142-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-CREB-1 (S142) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00577S142-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human CREB-1 around the phosphorylation site of S142.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00577S142-1 is reactive to CREB1 in Human, Mouse, Rat

Applications

A00577S142-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

36.688kDa

Background of CREB

Transcriptional regulator that binds to DNA as a dimer or as a tetramer, but not as a monomer. Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes By similarity. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Functions as a transcriptional activator and promotes the expression of otherwise tissue-specific self-antigens in the thymus, which is important for self tolerance and the avoidance of autoimmune reactions.

Nagamine K., Nat. Genet. 17:393-398(1997).
Aaltonen J., Nat. Genet. 17:399-403(1997).
Hattori M., Nature 405:311-319(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000

Validation Images & Assay Conditions

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Figure 3. Immunohistochemistry validation of CREB1 using Anti-Phospho-CREB-1 (S142) Antibody (A00577S142-1).

Immunohistochemical analysis of paraffin-embedded human lung cancer using Anti-Phospho-CREB-1 (S142) polyclonal antibody. Antibody was diluted at 1:100 (4°C

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Figure 4. Immunohistochemistry validation of CREB1 using Anti-Phospho-CREB-1 (S142) Antibody (A00577S142-1).

Immunohistochemical analysis of paraffin-embedded human lung cancer using Anti-Phospho-CREB-1 (S142) polyclonal antibody. Antibody was diluted at 1:100 (4°C

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Figure 1. Western blotting validation for Anti-Phospho-CREB-1 (S142) Antibody A00577S142-1

Western Blot (WB) analysis of HeLa cells using Anti-Phospho-CREB-1 (S142) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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Figure 2. Western blotting validation for Anti-Phospho-CREB-1 (S142) Antibody A00577S142-1

Western Blot (WB) analysis of HeLa cells using Anti-Phospho-CREB-1 (S142) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CREB1 (Source: Uniprot.org, NCBI)

Alternative Names

active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; pCREB; transactivator protein CREB1 CREB, CREB-1 cAMP responsive element binding protein 1 cyclic AMP-responsive element-binding protein 1|active transcription factor CREB|cAMP-response element-binding protein-1|cyclic adenosine 3,5-monophosphate response element binding protein|cyclic adenosine 3,5-monophosphate response element-binding protein CREB|transactivator protein

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In this infographic, you will see the following information for CREB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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