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Product Info Summary
| SKU: | PB9100 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CREB/CREB1 Antibody Picoband®
SKU/Catalog Number
PB9100
PB0127 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CREB/CREB1 Antibody Picoband® catalog # PB9100. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CREB/CREB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9100)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CREB recombinant protein (Position: M1-T177). Human CREB shares 99% and 98% amino acid (aa) sequences identity with mouse and rat CREB, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9100 is reactive to CREB1 in Human, Mouse, Rat
Observed Molecular Weight
43 kDa
Calculated molecular weight
36.7 kDa
Background of CREB1
CREB1, also known as cAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. It is mapped to 2q33.3. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9100 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human 293T whole cell, rat brain tissue, mouse brain tissue
FCM: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of CREB1 using anti-CREB1 antibody (PB9100).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB1 antigen affinity purified polyclonal antibody (PB9100) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CREB1 at approximately 43 kDa. The expected band size for CREB1 is at 35 kDa.
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Flow Cytometry analysis of 293T cells using anti-CREB1 antibody (PB9100).
Overlay histogram showing 293T cells stained with PB9100 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CREB1 Antibody (PB9100, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-CREB/CREB1 Antibody Picoband® (PB9100)
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Customer Q&As
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4 Customer Q&As for Anti-CREB/CREB1 Antibody Picoband®
Question
I am interested in using your anti-CREB/CREB1 antibody for secretory granule organization studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you for your inquiry. This PB9100 anti-CREB/CREB1 antibody is validated on rat thymus tissue, tissue lysate, brain tissue, ht1080 whole cell lysate. It is guaranteed to work for WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-24
Question
We have been able to see staining in rat testis. What should we do? Is anti-CREB/CREB1 antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
From what I have seen in literature testis does express CREB1. From what I have seen in Uniprot.org, CREB1 is expressed in lung, testis, eye, embryonic kidney, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2020-04-03
Question
Our team were well pleased with the WB result of your anti-CREB/CREB1 antibody. However we have been able to see positive staining in lung nucleus using this antibody. Is that expected? Could you tell me where is CREB1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
Based on literature, lung does express CREB1. Generally CREB1 expresses in nucleus. Regarding which tissues have CREB1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Testis, Pubmed ID: 15579595
Boster Scientific Support
Answered: 2019-11-05
Question
We are currently using anti-CREB/CREB1 antibody PB9100 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
The anti-CREB/CREB1 antibody (PB9100) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-13
