|Validated Species:||Human, Rat|
Data & Images
|Product Name||Anti-CREB Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Cyclic AMP-responsive element-binding protein 1(CREB1) detection. Tested with WB in Human;Rat.|
|Cite This Product||Anti-CREB Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9100)|
|Replacement Item||This antibody may replace the following items: sc-271932|sc-5539|sc-6835|sc-21735|sc-9905|sc-365671|sc-6835-R from Santa Cruz Biotechnology.|
|Validated Species||Human, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human CREB recombinant protein (Position: M1-T177). Human CREB shares 99% and 98% amino acid (aa) sequences identity with mouse and rat CREB, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclic AMP-responsive element-binding protein 1|
|Molecular Weight||36688 MW|
|Protein Function||Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.|
|Sequence Similarities||Belongs to the bZIP family.|
|Subcellular Localization||Nucleus .|
|Alternative Names||Cyclic AMP-responsive element-binding protein 1;CREB-1;cAMP-responsive element-binding protein 1;CREB1;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper| epigenetics and nuclear signaling|transcription factors| kits/ lysates/ other|elisa kits|signal transduction proteins elisa kits|phosphoprotein elisa kits||
Background for Cyclic AMP-responsive element-binding protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CREB Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CREB Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti CREB (PB9100) at 0.5ug/ml
WB: Recombinant Human CREB Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
All lanes: Anti CREB (PB9100) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 43KD
Observed bind size: 43KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,