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Product Info Summary
| SKU: | A02860T509 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-CRMP-2 (T509) DPYSL2 Antibody
SKU/Catalog Number
A02860T509
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-CRMP-2 (T509) DPYSL2 Antibody catalog # A02860T509. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-CRMP-2 (T509) DPYSL2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02860T509)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human CRMP-2 around the phosphorylation site of Thr509. AA range:475-524
Reactive Species
A02860T509 is reactive to DPYSL2 in Human, Mouse, Rat
Calculated molecular weight
62.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02860T509 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).
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Western Blot analysis of JK cells using Phospho-CRMP-2 (T509) Polyclonal Antibody
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Western blot analysis of lysates from HT29 cells treated with heat shock, using CRMP-2 (Phospho-Thr509) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using CRMP-2 (Phospho-Thr509) Antibody. The picture on the right is blocked with the phospho peptide.
Specific Publications For Anti-Phospho-CRMP-2 (T509) DPYSL2 Antibody (A02860T509)
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