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Product Info Summary
| SKU: | P01860 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Phospho-EphA4 (Y596) Antibody
SKU/Catalog Number
P01860
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Phospho-EphA4 (Y596) Antibody catalog # P01860. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-EphA4 (Y596) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01860)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized phospho-peptide around the phosphorylation site of human EphA4 (phospho Tyr596)
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P01860 is reactive to EPHA4 in Human, Mouse
Observed Molecular Weight
52 kDa
Calculated molecular weight
109.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P01860 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of JK cells using Phospho-EphA4 (Y596) Polyclonal Antibody
Specific Publications For Anti-Phospho-EphA4 (Y596) Antibody (P01860)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Phospho-EphA4 (Y596) Antibody
Question
Please see the WB image, lot number and protocol we used for forebrain using anti-Phospho-EphA4 (Y596) antibody P01860. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
We need to test anti-Phospho-EphA4 (Y596) antibody P01860 on human forebrain for research purposes, then I may be interested in using anti-Phospho-EphA4 (Y596) antibody P01860 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
The products we sell, including anti-Phospho-EphA4 (Y596) antibody P01860, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-20
Question
Will P01860 anti-Phospho-EphA4 (Y596) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
It shows on the product datasheet, P01860 anti-Phospho-EphA4 (Y596) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for forebrain using anti-Phospho-EphA4 (Y596) antibody P01860. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-16
Question
Will anti-Phospho-EphA4 (Y596) antibody P01860 work for WB with forebrain?
Verified Customer
Verified customer
Asked: 2017-08-18
Answer
According to the expression profile of forebrain, EPHA4 is highly expressed in forebrain. So, it is likely that anti-Phospho-EphA4 (Y596) antibody P01860 will work for WB with forebrain.
Boster Scientific Support
Answered: 2017-08-18
Question
We are currently using anti-Phospho-EphA4 (Y596) antibody P01860 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on dog tissues as well?
K. Mangal
Verified customer
Asked: 2017-07-06
Answer
The anti-Phospho-EphA4 (Y596) antibody (P01860) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-06
Question
I was wanting to use your anti-Phospho-EphA4 (Y596) antibody for WB for human forebrain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human forebrain identification?
P. Banerjee
Verified customer
Asked: 2017-04-12
Answer
It shows on the product datasheet, P01860 anti-Phospho-EphA4 (Y596) antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human forebrain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-12
