Anti-GFAP Antibody (Monoclonal, G-A-5)

Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC-P, IHC-F, WB applications. This antibody reacts with Human, Pig, Rat. Cited in 102 publication(s).

Product Info Summary

SKU: MA1045
Size: 100μg/vial
Reactive Species: Human, Pig, Rat
Host: Mouse
Application: IF, IHC-P, IHC-F, WB

Product Name

Anti-GFAP Antibody (Monoclonal, G-A-5)

See all Gfap products

SKU/Catalog Number

MA1045

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC-P, IHC-F, WB applications. This antibody reacts with Human, Pig, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GFAP Antibody (Monoclonal, G-A-5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1045)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

G-A-5

Isotype

Mouse IgG1

Immunogen

GFAP from pig spinal cord.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1045 is reactive to Gfap in Human, Pig, Rat

Applications

MA1045 is guaranteed for IF, IHC-P, IHC-F, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, pig, rat
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, pig, rat, -
Immunofluorescence, 2μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Gfap (Source: Uniprot.Org, NCBI)

Uniprot ID

P47819

Gene ID

24387

Gene Name

Gfap

Full Name

Glial fibrillary acidic protein

Weight

49.957kDa

Superfamily

intermediate filament family

Alternative Names

FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein Gfap|glial fibrillary acidic protein|glial fibrillary acidic protein|glial fibrillary acidic protein alpha|glial fibrillary acidic protein delta|intermediate filament

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Gfap, check out the Gfap Infographic

Gfap infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Gfap: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1045 has been cited in 102 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xiang Z,Jiang X,Ji R,Yuan H.Enhanced expression of P2X4 purinoceptors in pyramidal neurons of the rat hippocampal CA1 region may be involved ischemia-reperfusion injury.Purinergic Signal.2021 May 9.doi:10.1007/s11302-021-09780-z.Epub ahead of print.PMID:33966147.
Species: Rat
MA1045 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION: 1:400

Zhu X,Yao Y, Yang J,Zhang C,Li X,Zhang A,Liu X,Zhang C,Gan G.ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model.Free Radic Biol Med.2021 May 6:S0891-584 9(21)00282-3.doi:10.1016/j.freeradbiomed.2021.05.001.Epub ahead of print.PMID:33965566.
Species: Mouse
MA1045 usage in article: APP:ICC, SAMPLE:CORPUS CALLOSUM TISSUE, DILUTION:1:100

Xiang S,Zhao D,Hao H,Wang XU,Li L,Yang T.α-Helical protein absorption at post-traumatic epileptic foci monitored by Fourier transform infrared mapping.J Biosci.2020;45:55.PMID:32345781.
Species: Rat
MA1045 usage in article: APP:IHC, SAMPLE:FRONTAL BRAIN LOBE, DILUTION:1:100

Yonghui Hou,Jiyao Luan,Tiancheng Deng et al.Tauroursodeoxycholic Acid Alleviates Secondary Injury in Spinal Cord Injury Mice Through Reducing Oxidative Stress, Apoptosis, and Inflammatory Response,07 April 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-361252/ v1]
Species: Mouse
MA1045 usage in article: APP:IF, SAMPLE:CORTICAL NEURONS, DILUTION:1:500

Moraes TR,Elisei LS,Malta IH,Galdino G.Participation of CXCL1 in the glial cells during neuropathic pain.Eur J Pharmacol.2020 May 15;875:173039.doi:10.1016/j.ejphar.2020.173039.Epub 2020 Feb 28.PMID:32119843.
Species: Rat

Zhang Y,Zhang M,Zhu W,Pan X,Wang Q,Gao X,Wang C,Zhang X,Liu Y,Li S,Sun H.Role of Elevated Thrombospondin-1 in Kainic Acid-Induced Status Epilepticus.Neurosci Bull.2020 Mar;36(3):263-276.doi:10.1007/s12264-019-00437-x.Epub 2019 Oct 29.PMID:31664678;PMCID:P
Species: Rat
MA1045 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS AND CORTEX, DILUTION:1:1000

Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
MA1045 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
MA1045 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
MA1045 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20

Zhou Feng,Shengyan Liu,Qianwei Chen,Qiang Tan,Jishu Xian,Hua Feng,Zhi Chen,Gang Li,uPA alleviates kaolin-induced hydrocephalus by promoting the release and activation of hepatocyte growth factor in rats, Neuroscience Letters,Volume 731,2020,135011,ISSN 03
Species: Rat

Luo Z,Wang J,Tang S,Zheng Y,Zhou X,Tian F,Xu Z.Dynamic-related protein 1 inhibitor eases epileptic seizures and can regulate equilibrative nucleoside transporter 1 expression.BMC Neurol.2020 Sep 22;20(1):353.doi:10.1186/s12883-020-01921-y.PMID:32962663;PM
Species: Rat
MA1045 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Wang X,Tong Y,Zhang J,Khan N,Zhang K,Bai H,Zhang Q,Chen Y.Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice.Oral Dis.2020 Aug 20.doi:10.1111/odi.13618.Epub ahead of print.PMID:32815656.
Species: Mouse
MA1045 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:50

Wang X,Zhou W,Li X,Ren J,Ji G,Du J,Tian W,Liu Q,Hao A. Graphene oxide suppresses the growth and malignancy of glioblastoma stem cell-like spheroids via epigenetic mechanisms. J Transl Med.2020 May 14;18(1):200. doi:10.1186/s12967-020-02359-z.PMID:32410622
Species: Human
MA1045 usage in article: APP:WB, SAMPLE:GSCS, DILUTION:1:1000

Li R,Wen Y,Wu B,He M,Zhang P,Zhang Q,Chen Y.MicroRNA-25-3p suppresses epileptiform discharges through inhibiting oxidative stress and apoptosis via targeting OXSR1 in neurons.Biochem Biophys Res Commun. 2020 Mar 19;523(4):859-866.doi:10.1016/j.bbrc.2020.
Species: Rat
MA1045 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUL NEURONS, DILUTION:NA

Deng Z,Wei Y,Yao Y,Gao S,Wang X.Let-7f promotes the differentiation of neural stem cells in rats.Am J Transl Res.2020 Sep 15;12(9):5752-5761.PMID:33042454;PMCID:PMC7540113.
Species: Rat
MA1045 usage in article: APP:IF, SAMPLE:NSCS, DILUTION:1:100

Zhu X,Yao Y,Yang J,Zhengxie J,Li X,Hu S,Zhang A,Dong J,Zhang C,Gan G.COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. Int Immunopharmacol. 2020 Oct;87:106801.doi: 10.1016/j.inti
Species: Mouse
MA1045 usage in article: APP:ICC, SAMPLE:BRAIN TISSUE, DILUTION:1:100

Zhou Z,Hou J,Mo Y,Ren M,Yang G,Qu Z,Hu Y.Geniposidic acid ameliorates spatial learning and memory deficits and alleviates neuroinflammation via inhibiting HMGB-1 and downregulating TLR4/2 signaling pathway in APP/PS1 mice.Eur J Pharmacol.2020 Feb 15;869:1
Species: Mouse
MA1045 usage in article: APP:IHC, SAMPLE:BRAIN SECTION, DILUTION:1:200

Han Y,Chen M,Wang H. Production of a SCID mouse model of medulloblastoma to explore the therapeutic value of targeting tumor driver genes.Exp Ther Med.2021 Feb;21(2):108.doi: 10.3892/etm.2020.9540.Epub 2020 Nov 27.PMID: 33335571;PMCID:PMC7739861.
Species: Human, Mouse
MA1045 usage in article: APP:IHC, SAMPLE:HUMAN SUBCUTANEOUS TUMOR AND MOUSE SUBCUTANEOUS TUMOR, DILUTION:1:1000

Neuroprotective Effects of Pycnogenol Against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Primary Rat Astrocytes via NF-%u03BAB and ERK1/2 MAPK %u2026

Tumor-associated microglia/macrophages enhance the invasion of glioma stem-like cells via TGF-%u03B21 signaling pathway

Local injection of bone morphogenetic protein 7 promotes neuronal regeneration and motor function recovery after acute spinal cord injury

Electroacupuncture Suppresses the NF-?B Signaling Pathway by Upregulating Cylindromatosis to Alleviate Inflammatory Injury in Cerebral Ischemia/Reperfusion Rats

Interactions between GHRH and GABAARs in the brains of patients with epilepsy and in animal models of epilepsy

Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats

Water-soluble ginseng oligosaccharides protect against scopolamine-induced cognitive impairment by functioning as an antineuroinflammatory agent

Reversion of malignant phenotypes of human glioblastoma cells by ?-elemene through ?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules

Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats

Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats

Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice

Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice

In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats

The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects

Cyclin-dependent kinase inhibitor flavopiridol promotes remyelination in a cuprizone induced demyelination model

Evaluation of the Combination of Methylprednisolone and Tranilast after Spinal Cord Injury in Rat Models

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells

GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat

The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis

Transplantation of Neural Stem Cells Cotreated with Thyroid Hormone and GDNF Gene Induces Neuroprotection in Rats of Chronic Experimental Allergic Encephalomyelitis

Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells

Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro

Histidine provides long-term neuroprotection after cerebral ischemia through promoting astrocyte migration

Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells

Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer?s Disease-Like Amyloid-? Pathology and Neuroinflammation in the Rat Hippocampus

Acrylamide neurotoxicity on the cerebrum of weaning rats

Optimal electroacupuncture frequency for maintaining astrocyte structural integrity in cerebral ischemia

Neuroprotective Effect of Kaempferol Glycosides against Brain Injury and Neuroinflammation by Inhibiting the Activation of NF-?B and STAT3 in Transient Focal Stroke

Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia

Wang Y, Huang N, Li H, Liu S, Chen X, Yu S, Wu N, Bian XW, Shen HY, Li C, Xiao L. Oncotarget. 2017 Jun 6;8(23):37511-37524. doi: 10.18632/oncotarget.16400. Promoting oligodendroglial-oriented differentiation of glioma stem cell: a repurposing of q...

Zhang R, Pei H, Ru L, Li H, Liu G. Biomed Rep. 2013 Nov;1(6):895-900. Epub 2013 Sep 6. Bone Morphogenetic Protein 7 Upregulates The Expression Of Nestin And Glial Fibrillary Acidic Protein In Rats With Cerebral Ischemia-Reperfusion Injury.

Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...

Li T, Mo X, Jiang Z, He W, Lu W, Zhang H, Zhang J, Zeng L, Yang B, Xiao H, Hu Z. Oxid Med Cell Longev. 2012;2012:945071. Doi: 10.1155/2012/945071. Epub 2012 Oct 12. Study Of ??b-Crystallin Expression In Gerbil Bcao Model Of Transient Global Cerebr...

Zhou H, Cheng X, Rao L, Li T, Duan Yy. Acta Biomater. 2013 May;9(5):6439-49. Doi: 10.1016/J.Actbio.2013.01.042. Epub 2013 Feb 9. Poly(3,4-Ethylenedioxythiophene)/Multiwall Carbon Nanotube Composite Coatings For Improving The Stability Of Microelec...

Zhao Bq, Guo Yr, Li Xl, Zang T, Qu Hy, Zhou Jp, Li Q, Sun Mj. Int J Pharm. 2011 Feb 28;405(1-2):1-8. Doi: 10.1016/J.Ijpharm.2010.11.018. Epub 2010 Nov 18. Amelioration Of Dementia Induced By A?? 22-35 Through Rectal Delivery Of Undecapeptide-Hegf ...

Zhang S, Xie R, Wan F, Ye F, Guo D, Lei T. Oncol Lett. 2013 Dec;6(6):1649-1655. Epub 2013 Oct 11. Identification Of U251 Glioma Stem Cells And Their Heterogeneous Stem-Like Phenotypes.

Wang H, Li C, Wang H, Mei F, Liu Z, Shen Hy, Xiao L. Neurosci Bull. 2013 Apr;29(2):251-9. Doi: 10.1007/S12264-013-1323-1. Epub 2013 Apr 5. Cuprizone-Induced Demyelination In Mice: Age-Related Vulnerability And Exploratory Behavior Deficit.

Xu Dh, Yan M, Li Hd, Fang Pf, Liu Yw. Eur J Pharmacol. 2012 Sep 5;690(1-3):68-76. Doi: 10.1016/J.Ejphar.2012.06.032. Epub 2012 Jun 30. Influence Of P-Glycoprotein On Brucine Transport At The In Vitro Blood-Brain Barrier.

Yu Cz, Liu Yp, Liu S, Yan M, Hu Sj, Song Xj. Eur J Pain. 2014 Jan;18(1):76-85. Doi: 10.1002/J.1532-2149.2013.00390.X. Epub 2013 Sep 5. Systematic Administration Of B Vitamins Attenuates Neuropathic Hyperalgesia And Reduces Spinal Neuron Injury Fol...

Bai Y, Xu G, Xu M, Li Q, Qin X. Int J Mol Med. 2014 Dec;34(6):1473-82. Doi: 10.3892/Ijmm.2014.1946. Epub 2014 Sep 24. Inhibition Of Src Phosphorylation Reduces Damage To The Blood-Brain Barrier Following Transient Focal Cerebral Ischemia In Rats.

Zhang L, Wei Ws, Li Yj, Wang Y. Neuropathology. 2011 Apr;31(2):112-21. Doi: 10.1111/J.1440-1789.2010.01137.X. A Rat Model Of Mild Cognitive Impairment Associated With Vascular Factor.

Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.

Huang F, Wang J, Chen A. J Huazhong Univ Sci Technolog Med Sci. 2006;26(2):206-10. Effects Of Co-Grafts Mesenchymal Stem Cells And Nerve Growth Factor Suspension In The Repair Of Spinal Cord Injury.

Ma H, Yu B, Kong L, Zhang Y, Shi Y. Neurochem Res. 2012 Jan;37(1):69-83. Doi: 10.1007/S11064-011-0584-1. Epub 2011 Sep 7. Neural Stem Cells Over-Expressing Brain-Derived Neurotrophic Factor (Bdnf) Stimulate Synaptic Protein Expression And Promote ...

Huo Sj, Li Yc, Xie J, Li Y, Raisman G, Zeng Yx, He Jr, Weng Ch, Yin Zq. Curr Eye Res. 2012 Aug;37(8):749-58. Doi: 10.3109/02713683.2012.697972. Epub 2012 Jun 12. Transplanted Olfactory Ensheathing Cells Reduce Retinal Degeneration In Royal College...

Chen Sw, Chen Yx, Zhang Xr, Qian H, Chen Wz, Xie Wf. Gene Ther. 2008 Nov;15(21):1424-35. Doi: 10.1038/Gt.2008.93. Epub 2008 May 29. Targeted Inhibition Of Platelet-Derived Growth Factor Receptor-Beta Subunit In Hepatic Stellate Cells Ameliorates H...

Hu Ww, Wang Z, Zhang Ss, Jiang L, Zhang J, Zhang X, Lei Qf, Park Hj, Fang Wj, Chen Z. Biomaterials. 2014 Aug;35(26):7386-97. Doi: 10.1016/J.Biomaterials.2014.05.026. Epub 2014 Jun 2. Morphology And Functions Of Astrocytes Cultured On Water-Repelle...

Chu H, Sun J, Xu H, Niu Z, Xu M. Neurol Res. 2012 Nov;34(9):871-88. Doi: 10.1179/1743132812Y.0000000085. Epub 2012 Aug 13. Effect Of Periaqueductal Gray Melanocortin 4 Receptor In Pain Facilitation And Glial Activation In Rat Model Of Chronic Cons...

Zhang Y, Sun Lg, Ye Lp, Wang B, Li Y. Toxicol Mech Methods. 2008 Jan;18(9):751-7. Doi: 10.1080/15376510802390908. Lead-Induced Stress Response In Endoplasmic Reticulum Of Astrocytes In Cns.

Huo Sj, Li Y, Raisman G, Yin Zq. Brain Res. 2011 Mar 25;1382:238-44. Doi: 10.1016/J.Brainres.2010.12.079. Epub 2011 Jan 6. Transplanted Olfactory Ensheathing Cells Reduce The Gliotic Injury Response Of M??ller Cells In A Rat Model Of Retinitis Pig...

Zhao Yy, Yuan Y, Chen Y, Jiang L, Liao Rj, Wang L, Zhang Xn, Ohtsu H, Hu Ww, Chen Z. Cns Neurosci Ther. 2015 May;21(5):454-62. Doi: 10.1111/Cns.12379. Epub 2015 Jan 24. Histamine Promotes Locomotion Recovery After Spinal Cord Hemisection Via Inhib...

Yuan H, Chen R, Wu L, Chen Q, Hu A, Zhang T, Wang Z, Zhu X. Mol Neurobiol. 2015 Apr;51(2):512-22. Doi: 10.1007/S12035-014-8717-6. Epub 2014 Apr 29. The Regulatory Mechanism Of Neurogenesis By Igf-1 In Adult Mice.

Wang R, Zhang X, Zhang J, Fan Y, Shen Y, Hu W, Chen Z. Plos One. 2012;7(5):E37574. Doi: 10.1371/Journal.Pone.0037574. Epub 2012 May 22. Oxygen-Glucose Deprivation Induced Glial Scar-Like Change In Astrocytes.

Zhang J, Gao Y, Shen Y, Li K. Indian J Med Res. 2009 Feb;129(2):164-9. Asymmetrical Release Of Interleukin-6 By Cultured Cerebral Cortical Astrocytes Treated With Lipopolysaccharide.

Shi Xh, Han J, Zhang J, Shi Q, Chen Jm, Xia Sl, Xie Zq, Shen Xj, Shan B, Lei Yj, Shi S, Zhou W, Zhang By, Gao C, Liu Yh, Song J, Guo Yj, Wang Dx, Xu Bl, Dong Xp. Infect Genet Evol. 2010 Mar;10(2):292-7. Doi: 10.1016/J.Meegid.2010.01.007. Epub 2010...

Zhang G, Zhao Z, Gao L, Deng J, Wang B, Xu D, Liu B, Qu Y, Yu J, Li J, Gao G. Pharmacol Biochem Behav. 2011 Jul;99(1):42-51. Doi: 10.1016/J.Pbb.2011.03.019. Epub 2011 Apr 1. Gypenoside Attenuates White Matter Lesions Induced By Chronic Cerebral Hy...

Ma H, Yu B, Kong L, Zhang Y, Shi Y. Mol Med Rep. 2011 Sep-Oct;4(5):849-56. Doi: 10.3892/Mmr.2011.510. Epub 2011 Jun 16. Transplantation Of Neural Stem Cells Enhances Expression Of Synaptic Protein And Promotes Functional Recovery In A Rat Model Of...

Zhang Gl, Deng Jp, Wang Bh, Zhao Zw, Li J, Gao L, Liu Bl, Xong Jr, Guo Xd, Yan Zq, Gao Gd. Behav Pharmacol. 2011 Oct;22(7):633-44. Doi: 10.1097/Fbp.0B013E32834Afef9. Gypenosides Improve Cognitive Impairment Induced By Chronic Cerebral Hypoperfusio...

Huile G, Shuaiqi P, Zhi Y, Shijie C, Chen C, Xinguo J, Shun S, Zhiqing P, Yu H. Biomaterials. 2011 Nov;32(33):8669-75. Doi: 10.1016/J.Biomaterials.2011.07.069. Epub 2011 Aug 16. A Cascade Targeting Strategy For Brain Neuroglial Cells Employing Nan...

Wu L, Miao S, Zou Lb, Wu P, Hao H, Tang K, Zeng P, Xiong J, Li Hh, Wu Q, Cai L, Ye Dy. J Mol Neurosci. 2012 Sep;48(1):185-200. Doi: 10.1007/S12031-012-9807-4. Epub 2012 Jun 3. Lipoxin A4 Inhibits 5-Lipoxygenase Translocation And Leukotrienes Biosy...

Chen Y, Wu X, Yu S, Lin X, Wu J, Li L, Zhao J, Zhao Y. Plos One. 2012;7(6):E40165. Doi: 10.1371/Journal.Pone.0040165. Epub 2012 Jun 29. Neuroprotection Of Tanshinone Iia Against Cerebral Ischemia/Reperfusion Injury Through Inhibition Of Macrophage...

Liu Lx, Zhou Xy, Li Cs, Liu Lq, Huang Sy, Zhou Sn. Neurol Sci. 2013 Sep;34(9):1671-8. Doi: 10.1007/S10072-013-1319-7. Epub 2013 Feb 13. Selenoprotein S Expression In The Rat Brain Following Focal Cerebral Ischemia.

Chen Z, Pan X, Georgakilas Ag, Chen P, Hu H, Yang Y, Tian S, Xia L, Zhang J, Cai X, Ge J, Yu K, Zhuang J. Cancer Lett. 2013 Aug 19;336(2):281-9. Doi: 10.1016/J.Canlet.2013.03.015. Epub 2013 Mar 21. Tetramethylpyrazine (Tmp) Protects Cerebral Neuro...

Chen X, Chen L, Chen J, Hu W, Gao H, Xie B, Wang X, Yin Z, Li S, Wang X. Brain Res. 2013 Nov 13;1538:151-8. Doi: 10.1016/J.Brainres.2013.02.025. Epub 2013 Mar 5. Adam17 Promotes U87 Glioblastoma Stem Cell Migration And Invasion.

Yu Q, Guo Z, Liu X, Ouyang Q, He C, Burnstock G, Yuan H, Xiang Z. Purinergic Signal. 2013 Dec;9(4):663-75. Doi: 10.1007/S11302-013-9379-Y. Epub 2013 Jul 23. Block Of P2X7 Receptors Could Partly Reverse The Delayed Neuronal Death In Area Ca1 Of The...

Sun Yx, Cong Yl, Liu Y, Jin B, Si L, Wang Ab, Cai H, Che Gy, Tang B, Wang Cf, Li Zy, Zhang Xm. Eur J Pharmacol. 2014 Oct 5;740:552-9. Doi: 10.1016/J.Ejphar.2014.06.032. Epub 2014 Jun 27. Schisandrin A And B Affect Subventricular Zone Neurogenesis ...

Xu X, Yang X, Xiong Y, Gu J, He C, Hu Y, Xiao F, Chen G, Wang X. J Neurochem. 2015 Apr;133(1):134-43. Doi: 10.1111/Jnc.13052. Epub 2015 Feb 26. Increased Expression Of Receptor For Activated C Kinase 1 In Temporal Lobe Epilepsy.

Lan Lf, Zheng L, Yang X, Ji Xt, Fan Yh, Zeng Js. Cns Neurosci Ther. 2015 May;21(5):410-6. Doi: 10.1111/Cns.12374. Epub 2015 Jan 22. Peroxisome Proliferator-Activated Receptor-?? Agonist Pioglitazone Ameliorates White Matter Lesion And Cognitive Im...

Zhang L, Li H, Zeng S, Chen L, Fang Z, Huang Q. Neurosci Lett. 2015 Mar 30;591:30-4. Doi: 10.1016/J.Neulet.2015.02.023. Epub 2015 Feb 11. Long-Term Tracing Of The Brdu Label-Retaining Cells In Adult Rat Brain.

Zhu X, Dong J, Shen K, Bai Y, Zhang Y, Lv X, Chao J, Yao H. Brain Res Bull. 2015 May;114:70-8. Doi: 10.1016/J.Brainresbull.2015.04.002. Epub 2015 Apr 17. Nmda Receptor Nr2B Subunits Contribute To Ptz-Kindling-Induced Hippocampal Astrocytosis And O...

Shi J, Ma Y, Zheng M, Ruan Z, Liu J, Tian S, Zhang D, He X, Li G. Toxicol Ind Health. 2012 Feb;28(1):10-20. Doi: 10.1177/0748233711401264. Epub 2011 Mar 28. Effect Of Sub-Acute Exposure To Acrylamide On Gabaergic Neurons And Astrocytes In Weaning ...

Li F, Miao Zn, Xu Yy, Zheng Sy, Qin Md, Gu Yz, Zhang Xg. Mol Med Rep. 2012 Sep;6(3):625-30. Doi: 10.3892/Mmr.2012.968. Epub 2012 Jun 26. Transplantation Of Human Amniotic Mesenchymal Stem Cells In The Treatment Of Focal Cerebral Ischemia.

Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...

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16 Customer Q&As for Anti-GFAP Antibody (Monoclonal, G-A-5)

Question

We are currently using anti-GFAP antibody (Monoclonal, G-A-5) MA1045 for pig tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, pig, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The anti-GFAP antibody (Monoclonal, G-A-5) (MA1045) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-31

Question

Is this MA1045 anti-GFAP antibody (Monoclonal, G-A-5) reactive to the isotypes of GFAP?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

The immunogen of MA1045 anti-GFAP antibody (Monoclonal, G-A-5) is GFAP from pig spinal cord. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-GFAP antibody (Monoclonal, G-A-5) MA1045. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

you antibody to test anti-GFAP antibody (Monoclonal, G-A-5) MA1045 on rat blood for research purposes, then I may be interested in using anti-GFAP antibody (Monoclonal, G-A-5) MA1045 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

The products we sell, including anti-GFAP antibody (Monoclonal, G-A-5) MA1045, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-29

Question

Is a blocking peptide available for product anti-GFAP antibody (Monoclonal, G-A-5) (MA1045)?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

We do provide the blocking peptide for product anti-GFAP antibody (Monoclonal, G-A-5) (MA1045). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-03

Question

We have seen staining in rat brain thalamus. Any tips? Is anti-GFAP antibody (Monoclonal, G-A-5) supposed to stain brain thalamus positively?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

From what I have seen in literature brain thalamus does express GFAP. From what I have seen in Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-09-26

Question

I was wanting to use your anti-GFAP antibody (Monoclonal, G-A-5) for IHC-P for rat blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat blood identification?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

It shows on the product datasheet, MA1045 anti-GFAP antibody (Monoclonal, G-A-5) has been tested for IF, IHC-P, IHC-F, WB on human, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-05

Question

I have attached the WB image, lot number and protocol we used for blood using anti-GFAP antibody (Monoclonal, G-A-5) MA1045. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-03

Question

We bought anti-GFAP antibody (Monoclonal, G-A-5) for IF on kidney a few years ago. I am using rat, and We intend to use the antibody for IHC-P next. My question regards examining kidney as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

H. Roberts

Verified customer

Asked: 2018-10-25

Answer

I have checked the website and datasheets of our anti-GFAP antibody (Monoclonal, G-A-5) and it seems that MA1045 has been validated on rat in both IF and IHC-P. Thus MA1045 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-25

Question

My question regarding product MA1045, anti-GFAP antibody (Monoclonal, G-A-5). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1045 anti-GFAP antibody (Monoclonal, G-A-5), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-05

Question

Is there a BSA free version of anti-GFAP antibody (Monoclonal, G-A-5) MA1045 available?

Verified Customer

Verified customer

Asked: 2017-09-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP antibody (Monoclonal, G-A-5) MA1045 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-18

Question

We are interested in using your anti-GFAP antibody (Monoclonal, G-A-5) for r-rno-9613829; chaperone mediated autophagy studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-08-03

Answer

I appreciate your inquiry. This MA1045 anti-GFAP antibody (Monoclonal, G-A-5) is validated on rat brain tissue. It is guaranteed to work for IF, IHC-P, IHC-F, WB in human, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-03

Question

Would MA1045 anti-GFAP antibody (Monoclonal, G-A-5) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Jackson

Verified customer

Asked: 2017-07-13

Answer

It shows on the product datasheet, MA1045 anti-GFAP antibody (Monoclonal, G-A-5) as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-13

Question

I see that the anti-GFAP antibody (Monoclonal, G-A-5) MA1045 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-05-17

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-05-17

Question

My colleagues were happy with the WB result of your anti-GFAP antibody (Monoclonal, G-A-5). However we have seen positive staining in fetal brain cortex cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?

A. Li

Verified customer

Asked: 2016-10-11

Answer

From literature, fetal brain cortex does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2016-10-11

Question

Will anti-GFAP antibody (Monoclonal, G-A-5) MA1045 work for IHC-P with blood?

L. Wu

Verified customer

Asked: 2015-12-30

Answer

According to the expression profile of blood, GFAP is highly expressed in blood. So, it is likely that anti-GFAP antibody (Monoclonal, G-A-5) MA1045 will work for IHC-P with blood.

Boster Scientific Support

Answered: 2015-12-30

Size

Total: $280

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MA1045
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

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