Anti-GFAP Antibody (Monoclonal, G-A-5)

Gfap antibody

Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: MA1045
Size: 100 μg/vial
Reactive Species: Human, Mouse, Pig, Rat
Host: Mouse
Application: IF, IHC, IHC-F, WB

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Product Name

Anti-GFAP Antibody (Monoclonal, G-A-5)

View all Gfap Antibodies

SKU/Catalog Number

MA1045

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GFAP Antibody (Monoclonal, G-A-5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1045)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

G-A-5

Isotype

Mouse IgG1

Immunogen

GFAP from pig spinal cord.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1045 is reactive to Gfap in Human, Mouse, Pig, Rat

Applications

MA1045 is guaranteed for IF, IHC, IHC-F, WB Boster Guarantee

Background of Gfap

Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.5-1μg/ml, Human, mouse, pig, rat
Immunohistochemistry (Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, pig, rat, -
Immunofluorescence, 2μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Gfap (Source: Uniprot.org, NCBI)

Uniprot ID

P47819

Gene ID

24387

Gene Name

Gfap

Full Name

Glial fibrillary acidic protein

Weight

49.957kDa

Superfamily

intermediate filament family

Alternative Names

FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein Gfap|glial fibrillary acidic protein|glial fibrillary acidic protein|glial fibrillary acidic protein alpha|glial fibrillary acidic protein delta|intermediate filament

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Gfap, check out the Gfap Infographic

Gfap infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Gfap: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

MA1045 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

Xiang Z,Jiang X,Ji R,Yuan H.Enhanced expression of P2X4 purinoceptors in pyramidal neurons of the rat hippocampal CA1 region may be involved ischemia-reperfusion injury.Purinergic Signal.2021 May 9.doi:10.1007/s11302-021-09780-z.Epub ahead of print.PMID:33966147.
Species: Rat
MA1045 usage in article: APP:IF, SAMPLE:BRAIN TISSUE, DILUTION: 1:400

Zhu X,Yao Y, Yang J,Zhang C,Li X,Zhang A,Liu X,Zhang C,Gan G.ADAM10 suppresses demyelination and reduces seizure susceptibility in cuprizone-induced demyelination model.Free Radic Biol Med.2021 May 6:S0891-584 9(21)00282-3.doi:10.1016/j.freeradbiomed.2021.05.001.Epub ahead of print.PMID:33965566.
Species: Mouse
MA1045 usage in article: APP:ICC, SAMPLE:CORPUS CALLOSUM TISSUE, DILUTION:1:100

Xiang S,Zhao D,Hao H,Wang XU,Li L,Yang T.α-Helical protein absorption at post-traumatic epileptic foci monitored by Fourier transform infrared mapping.J Biosci.2020;45:55.PMID:32345781.
Species: Rat
MA1045 usage in article: APP:IHC, SAMPLE:FRONTAL BRAIN LOBE, DILUTION:1:100

Tauroursodeoxycholic Acid Alleviates Secondary Injury in Spinal Cord Injury Mice Through Reducing Oxidative Stress

Authors:Yonghui Hou,Jiyao Luan,Tiancheng Deng et al.


Species: Mouse
MA1045 usage in article: APP:IF, SAMPLE:CORTICAL NEURONS, DILUTION:1:500

Moraes TR,Elisei LS,Malta IH,Galdino G.Participation of CXCL1 in the glial cells during neuropathic pain.Eur J Pharmacol.2020 May 15;875:173039.doi:10.1016/j.ejphar.2020.173039.Epub 2020 Feb 28.PMID:32119843.
Species: Rat

Zhang Y,Zhang M,Zhu W,Pan X,Wang Q,Gao X,Wang C,Zhang X,Liu Y,Li S,Sun H.Role of Elevated Thrombospondin-1 in Kainic Acid-Induced Status Epilepticus.Neurosci Bull.2020 Mar;36(3):263-276.doi:10.1007/s12264-019-00437-x.Epub 2019 Oct 29.PMID:31664678;PMCID:P
Species: Rat
MA1045 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS AND CORTEX, DILUTION:1:1000

Zhang LY,Jin QQ,Hölscher C,Li L.Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model. Neural Regen Res 2021;16:1660-70
Species: Human,Rat
MA1045 usage in article: APP:IF, SAMPLE:SN TISSUE, DILUTION:1:100

Jiangong Wang,Bin Liu,Yong Xu et al. Inhibition of Histamine H3 receptor Attenuates Neuroinflammation and Cognitive Impairments in Alzheimer’s Disease via activating CREB Pathway,16 December 2020, PREPRINT (Version 1) available at Research Square [https:/
Species: Mouse
MA1045 usage in article: APP:IHC, SAMPLE:BRAIN TISSUE, DILUTION:1:200

Ahmed S. Ahmed,JAK-1/STAT-3 pathway mediated role in aging cerebellar cortex degenerative changes of albino wistar rats, Translational Research in Anatomy, 2020,100089,ISSN 2214-854X, https://doi.org/10. 1016/j.tria.2020.100089.
Species: Rat
MA1045 usage in article: APP:IHC, SAMPLE:CEREBELLAR CORTEX, DILUTION:1:20

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16 Customer Q&As for Anti-GFAP Antibody (Monoclonal, G-A-5)

Question

We are currently using anti-GFAP antibody (Monoclonal, G-A-5) MA1045 for pig tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, pig, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

The anti-GFAP antibody (Monoclonal, G-A-5) (MA1045) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-31

Question

Is this MA1045 anti-GFAP antibody (Monoclonal, G-A-5) reactive to the isotypes of GFAP?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

The immunogen of MA1045 anti-GFAP antibody (Monoclonal, G-A-5) is GFAP from pig spinal cord. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-GFAP antibody (Monoclonal, G-A-5) MA1045. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

you antibody to test anti-GFAP antibody (Monoclonal, G-A-5) MA1045 on rat blood for research purposes, then I may be interested in using anti-GFAP antibody (Monoclonal, G-A-5) MA1045 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

The products we sell, including anti-GFAP antibody (Monoclonal, G-A-5) MA1045, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-11-29

Question

Is a blocking peptide available for product anti-GFAP antibody (Monoclonal, G-A-5) (MA1045)?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

We do provide the blocking peptide for product anti-GFAP antibody (Monoclonal, G-A-5) (MA1045). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-03

Question

We have seen staining in rat brain thalamus. Any tips? Is anti-GFAP antibody (Monoclonal, G-A-5) supposed to stain brain thalamus positively?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

From what I have seen in literature brain thalamus does express GFAP. From what I have seen in Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-09-26

Question

I was wanting to use your anti-GFAP antibody (Monoclonal, G-A-5) for IHC-P for rat blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat blood identification?

Verified Customer

Verified customer

Asked: 2019-08-05

Answer

It shows on the product datasheet, MA1045 anti-GFAP antibody (Monoclonal, G-A-5) has been tested for IF, IHC-P, IHC-F, WB on human, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-05

Question

I have attached the WB image, lot number and protocol we used for blood using anti-GFAP antibody (Monoclonal, G-A-5) MA1045. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-03

Question

We bought anti-GFAP antibody (Monoclonal, G-A-5) for IF on kidney a few years ago. I am using rat, and We intend to use the antibody for IHC-P next. My question regards examining kidney as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

H. Roberts

Verified customer

Asked: 2018-10-25

Answer

I have checked the website and datasheets of our anti-GFAP antibody (Monoclonal, G-A-5) and it seems that MA1045 has been validated on rat in both IF and IHC-P. Thus MA1045 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-25

Question

My question regarding product MA1045, anti-GFAP antibody (Monoclonal, G-A-5). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-05

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1045 anti-GFAP antibody (Monoclonal, G-A-5), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-05

Question

Is there a BSA free version of anti-GFAP antibody (Monoclonal, G-A-5) MA1045 available?

Verified Customer

Verified customer

Asked: 2017-09-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP antibody (Monoclonal, G-A-5) MA1045 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-09-18

Question

We are interested in using your anti-GFAP antibody (Monoclonal, G-A-5) for r-rno-9613829; chaperone mediated autophagy studies. Has this antibody been tested with western blotting on rat brain tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-08-03

Answer

I appreciate your inquiry. This MA1045 anti-GFAP antibody (Monoclonal, G-A-5) is validated on rat brain tissue. It is guaranteed to work for IF, IHC-P, IHC-F, WB in human, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-08-03

Question

Would MA1045 anti-GFAP antibody (Monoclonal, G-A-5) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Jackson

Verified customer

Asked: 2017-07-13

Answer

It shows on the product datasheet, MA1045 anti-GFAP antibody (Monoclonal, G-A-5) as been validated on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-13

Question

I see that the anti-GFAP antibody (Monoclonal, G-A-5) MA1045 works with IHC-P, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-05-17

Answer

You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-05-17

Question

My colleagues were happy with the WB result of your anti-GFAP antibody (Monoclonal, G-A-5). However we have seen positive staining in fetal brain cortex cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?

A. Li

Verified customer

Asked: 2016-10-11

Answer

From literature, fetal brain cortex does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2016-10-11

Question

Will anti-GFAP antibody (Monoclonal, G-A-5) MA1045 work for IHC-P with blood?

L. Wu

Verified customer

Asked: 2015-12-30

Answer

According to the expression profile of blood, GFAP is highly expressed in blood. So, it is likely that anti-GFAP antibody (Monoclonal, G-A-5) MA1045 will work for IHC-P with blood.

Boster Scientific Support

Answered: 2015-12-30

Size

Conjugation

Total: $315

SKU:MA1045

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MA1045
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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