SKU MA1045
Size 100μg/vial
Reactivity Human, Pig, Rat
Clonality Monoclonal
Clone Number G-A-5
Host Mouse
Ig Isotype Mouse IgG1
Applications IHC, WB

Overview

Product Name Anti-GFAP Antibody (Monoclonal, G-A-5)
SKU/Catalog Number MA1045
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for GFAP, glial fibrillary acidic protein (GFAP) detection. Tested with WB, IHC-P, IHC-F in Human;pig;rat. No cross reactivity with other proteins.
Cite This Product Anti-GFAP Antibody (Monoclonal, G-A-5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1045)
Host Mouse
Immunogen GFAP from pig spinal cord.
Reactivity Human, Pig, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.4-1μg/ml, Human, pig, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, pig, rat, -
Western blot, 0.5-1μg/ml, Human, pig, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1045 1 IHC anti gfap antibody monoclonal g a 5.jpg

Anti- GFAP antibody, MA1045, IHC(P)
IHC(P): Rat Brain Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P47819
Gene Name Gfap
Protein Name Glial fibrillary acidic protein
Tissue Specificity Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment. .
Alternative Names Glial fibrillary acidic protein;GFAP;Gfap;
Subcellular Localization Cytoplasm . Associated with intermediate filaments. .
Molecular Weight 49957 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Background Glial fibrillary acidic protein(GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Order Product (MA1045)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280
Ships in 5-7 business days.

Troubleshooting

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Publications

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Inhibition of P2X7 receptors improves outcomes after traumatic brain injury in rats
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Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
Effect of Corilagin on the Proliferation and NF-?B in U251 Glioblastoma Cells and U251 Glioblastoma Stem-Like Cells
The hGFAP-driven conditional TSPO knockout is protective in a mouse model of multiple sclerosis
Re-infection of the prion from the scrapie?infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c
Effect of siRNA-Livin on drug resistance to chemotherapy in glioma U251 cells and CD133+ stem cells
Effects of nerve growth factor and basic fibroblast growth factor dual gene modification on rat bone marrow mesenchymal stem cell differentiation into neuron-like cells in vitro
Reversion of malignant phenotypes of human glioblastoma cells by?-elemene through?-catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules
Different methods for inducing adipose-derived stem cells to differentiate into Schwann-like cells
Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer?s Disease-Like Amyloid-? Pathology and Neuroinflammation in the Rat Hippocampus
Brain Localization and Neurotoxicity Evaluation of Polysorbate 80-Modified Chitosan Nanoparticles in Rats
Acrylamide neurotoxicity on the cerebrum of weaning rats
Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury
Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene
Ipsilateral versus bilateral limb-training in promoting the proliferation and differentiation of endogenous neural stem cells following cerebral infarction in rats
Inhibition of Src phosphorylation reduces damage to the blood-brain barrier following transient focal cerebral ischemia in rats
Bone morphogenetic protein 7 upregulates the expression of nestin and glial fibrillary acidic protein in rats with cerebral ischemia-reperfusion injury
Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection
Study of ?B-Crystallin Expression in Gerbil BCAO Model of Transient Global Cerebral Ischemia
Neuroprotection of Tanshinone IIA against Cerebral Ischemia/Reperfusion Injury through Inhibition of Macrophage Migration Inhibitory Factor in Rats
Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia
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Zhang L, Wei Ws, Li Yj, Wang Y. Neuropathology. 2011 Apr;31(2):112-21. Doi: 10.1111/J.1440-1789.2010.01137.X. A Rat Model Of Mild Cognitive Impairment Associated With Vascular Factor.
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Zhang Y, Sun Lg, Ye Lp, Wang B, Li Y. Toxicol Mech Methods. 2008 Jan;18(9):751-7. Doi: 10.1080/15376510802390908. Lead-Induced Stress Response In Endoplasmic Reticulum Of Astrocytes In Cns.
Huo Sj, Li Y, Raisman G, Yin Zq. Brain Res. 2011 Mar 25;1382:238-44. Doi: 10.1016/J.Brainres.2010.12.079. Epub 2011 Jan 6. Transplanted Olfactory Ensheathing Cells Reduce The Gliotic Injury Response Of M??ller Cells In A Rat Model Of Retinitis Pigm...
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Wang R, Zhang X, Zhang J, Fan Y, Shen Y, Hu W, Chen Z. Plos One. 2012;7(5):E37574. Doi: 10.1371/Journal.Pone.0037574. Epub 2012 May 22. Oxygen-Glucose Deprivation Induced Glial Scar-Like Change In Astrocytes.
Zhang J, Gao Y, Shen Y, Li K. Indian J Med Res. 2009 Feb;129(2):164-9. Asymmetrical Release Of Interleukin-6 By Cultured Cerebral Cortical Astrocytes Treated With Lipopolysaccharide.
Shi Xh, Han J, Zhang J, Shi Q, Chen Jm, Xia Sl, Xie Zq, Shen Xj, Shan B, Lei Yj, Shi S, Zhou W, Zhang By, Gao C, Liu Yh, Song J, Guo Yj, Wang Dx, Xu Bl, Dong Xp. Infect Genet Evol. 2010 Mar;10(2):292-7. Doi: 10.1016/J.Meegid.2010.01.007. Epub 2010...
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Lan Lf, Zheng L, Yang X, Ji Xt, Fan Yh, Zeng Js. Cns Neurosci Ther. 2015 May;21(5):410-6. Doi: 10.1111/Cns.12374. Epub 2015 Jan 22. Peroxisome Proliferator-Activated Receptor-?? Agonist Pioglitazone Ameliorates White Matter Lesion And Cognitive Imp...
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Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gfap antibody, astrocyte antibody, glial fibrillary acidic protein antibody