Click image to see more details
Product Info Summary
| SKU: | P00252 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00252
BM4401 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody catalog # P00252. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00252)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ECG-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FoxO3a
Reactive Species
P00252 is reactive to FOXO3 in Human, Mouse, Rat
Observed Molecular Weight
80-100 kDa
Calculated molecular weight
71.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00252 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human T47D whole cell, human K562 whole cell, human CACO-2 whole cell, human PC-3 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: human uterus cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Phospho-FoxO3a (S253) using anti-Phospho-FoxO3a (S253) antibody (P00252).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human T47D whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human CACO-2 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-FoxO3a (S253) antigen affinity purified monoclonal antibody (P00252) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Phospho-FoxO3a (S253) at approximately 80-100 kDa. The expected band size for Phospho-FoxO3a (S253) is at 71 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human uterus cancer, using Phospho-FoxO3a (S253) Antibody.
Specific Publications For Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody (P00252)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody
Question
Will P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
You can see on the product datasheet, P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-20
Question
Is there a BSA free version of anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 available?
D. Lewis
Verified customer
Asked: 2020-02-18
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-21
Question
I was wanting to use your anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody for WB for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
You can see on the product datasheet, P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-03
Question
Would anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
According to the expression profile of cervix carcinoma erythroleukemia, FOXO3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-09-17
Question
Is this P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody reactive to the isotypes of FOXO3?
D. Baker
Verified customer
Asked: 2019-07-16
Answer
The immunogen of P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody is A synthesized peptide derived from human FoxO3a. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-16
Question
We have been able to see staining in human muscle placenta. Any tips? Is anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody supposed to stain muscle placenta positively?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
From what I have seen in literature muscle placenta does express FOXO3. From what I have seen in Uniprot.org, FOXO3 is expressed in trabecular bone tissue, rhabdomyosarcoma, stomach, muscle placenta, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FOXO3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Muscle, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Stomach, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-11-22
Question
Can you help my question with product P00252, anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-07
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-07
Question
We are currently using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody (P00252) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-09
Question
Will anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 work on monkey WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2017-10-19
Answer
Our lab technicians have not tested anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-19
Question
We need to test anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-15
Answer
The products we sell, including anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-15
Question
My boss were content with the WB result of your anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia cytosol using this antibody. Is that expected? Could you tell me where is FOXO3 supposed to be expressed?
R. Brown
Verified customer
Asked: 2014-09-01
Answer
According to literature, cervix carcinoma erythroleukemia does express FOXO3. Generally FOXO3 expresses in cytoplasm, cytosol. Regarding which tissues have FOXO3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Muscle, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Stomach, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-09-01
Question
Is a blocking peptide available for product anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody (P00252)?
F. Miller
Verified customer
Asked: 2013-09-18
Answer
We do provide the blocking peptide for product anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody (P00252). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2013-09-18
Question
I see that the anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 works with WB, what is the protocol used to produce the result images on the product page?
B. Baker
Verified customer
Asked: 2013-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2013-08-16
