Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody

Boster Bio Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody catalog # P00252. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: P00252
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody

See all FOXO3 products

SKU/Catalog Number

P00252

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody catalog # P00252. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00252)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ECG-6

Immunogen

A synthesized peptide derived from human FoxO3a

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00252 is reactive to FOXO3 in Human, Mouse, Rat

Applications

P00252 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For FOXO3 (Source: Uniprot.Org, NCBI)

Uniprot ID

O43524

Gene ID

2309

Gene Name

FOXO3

Full Name

Forkhead box protein O3

Weight

71.277kDa

Alternative Names

AF6q21 protein; AF6q21; DKFZp781A0677; FKHRL1; FKHRL1MGC31925; FKHRL1P2; forkhead box O3; forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; Forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO2; FoxO3; FOXO3A; FOXO3Aforkhead box O3A; MGC12739 FOXO3 AF6q21, FKHRL1, FKHRL1P2, FOXO2A, FOXO3 forkhead box O3 forkhead box protein O3|forkhead box O3A|forkhead homolog (rhabdomyosarcoma) like 1|forkhead in rhabdomyosarcoma-like 1|forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on FOXO3, check out the FOXO3 Infographic

FOXO3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for FOXO3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for P00252

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15 Customer Q&As for Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody

Question

Will P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-20

Answer

You can see on the product datasheet, P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-20

Question

Is there a BSA free version of anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 available?

D. Lewis

Verified customer

Asked: 2020-02-18

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-18

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-11

Question

I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-01-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-21

Question

I was wanting to use your anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody for WB for mouse cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

You can see on the product datasheet, P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-03

Question

Would anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 work for WB with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

According to the expression profile of cervix carcinoma erythroleukemia, FOXO3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2019-09-17

Question

Is this P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody reactive to the isotypes of FOXO3?

D. Baker

Verified customer

Asked: 2019-07-16

Answer

The immunogen of P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody is A synthesized peptide derived from human FoxO3a. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-16

Question

We have been able to see staining in human muscle placenta. Any tips? Is anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody supposed to stain muscle placenta positively?

Verified Customer

Verified customer

Asked: 2018-11-22

Answer

From what I have seen in literature muscle placenta does express FOXO3. From what I have seen in Uniprot.org, FOXO3 is expressed in trabecular bone tissue, rhabdomyosarcoma, stomach, muscle placenta, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have FOXO3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Muscle, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Stomach, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-11-22

Question

Can you help my question with product P00252, anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-09-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00252 anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-09-07

Question

We are currently using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

The anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody (P00252) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-09

Question

Will anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 work on monkey WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2017-10-19

Answer

Our lab technicians have not tested anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey leukemic t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-10-19

Question

We need to test anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-15

Answer

The products we sell, including anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-15

Question

My boss were content with the WB result of your anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody. However we have observed positive staining in cervix carcinoma erythroleukemia cytosol using this antibody. Is that expected? Could you tell me where is FOXO3 supposed to be expressed?

R. Brown

Verified customer

Asked: 2014-09-01

Answer

According to literature, cervix carcinoma erythroleukemia does express FOXO3. Generally FOXO3 expresses in cytoplasm, cytosol. Regarding which tissues have FOXO3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18220336, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Muscle, and Placenta, Pubmed ID: 15489334
Rhabdomyosarcoma, Pubmed ID: 9479491
Stomach, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2014-09-01

Question

Is a blocking peptide available for product anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody (P00252)?

F. Miller

Verified customer

Asked: 2013-09-18

Answer

We do provide the blocking peptide for product anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody (P00252). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-09-18

Question

I see that the anti-Phospho-FoxO3a (S253) Rabbit Monoclonal antibody P00252 works with WB, what is the protocol used to produce the result images on the product page?

B. Baker

Verified customer

Asked: 2013-08-16

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2013-08-16

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
P00252
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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