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- Table of Contents
FOXO3 antibody (P00252) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunohistochemical analysis of paraffin-embedded human uterus cancer, using Phospho-FoxO3a (S253) Antibody.
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Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody
FOXO3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
FOXO3 antibody was positively detected by WB in:
human T47D whole cell, human K562 whole cell, human CACO-2 whole cell, human PC-3 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FOXO3 antibody was positively detected by IHC in:
human uterus cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Mouse
Tested
Product Information
Product Name
Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody
View all FOXO3 Antibodies
View all FOXO3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ECG-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human FoxO3a
Observed Molecular Weight
80-100 kDa
Calculated Molecular Weight
71.3 kDa
Cite This Product
Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00252)
Protocols
Western Blot protocol for FOXO3 antibody
Immunohistochemistry (IHC) protocol for FOXO3 antibody
Publications
Application:
Species:
Chondroitin sulfate A-selenium nanoparticles protect chondrocytes from T-2 toxin-induced oxidative stress and mitochondrial dysfunction through activating autophagy by the SIRT1-AMPK-FOXO3 pathway
Published: 2025-08-04
Journal: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Application: WB
Impact Factor: 6.10
Species: Human
PMID: 40763514
(2E)-1-(2,4,6-Trimethoxyphenyl)-3-(4-chlorophenyl)prop-2-en-1-one, a Chalcone Derivative, Promotes Apoptosis by Suppressing RAS-ERK and AKT/FOXO3a Pathways in Hepatocellular Carcinoma Cells
Published: 2023-06-14
Journal: CHEMISTRY & BIODIVERSITY
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 37314937
TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer’s disease mice
Published: 2020-10-16
Journal: Aging-US
Application: IF,WB
Impact Factor: 4.83
Species: Mouse
PMID: 33065553
Fucoxanthin Alleviates Oxidative Stress through Akt/Sirt1/FoxO3α Signaling to Inhibit HG-Induced Renal Fibrosis in GMCs
Published: 2019-12-12
Journal: Marine Drugs
Application: WB,IF
Impact Factor: 3.77
Species: Rat
PMID: 31842414
Review
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Product Promise
We promise Anti-Phospho-FoxO3a (S253) Rabbit Monoclonal Antibody performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P00252
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026