Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody

HSF1 antibody

Boster Bio Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody catalog # P00250. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: P00250
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody

View all HSF1 Antibodies

SKU/Catalog Number

P00250

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody catalog # P00250. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00250)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BHI-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Phospho-HSF1 (S326)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

P00250 is reactive to HSF1 in Human

Reconstitution

Observed Molecular Weight

80 kDa

Calculated molecular weight

57260 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

P00250 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Positive Control

WB: human Hela whole cell, human K562 whole cell, human HepG2 whole cell, human MCF-7 whole cell
IHC: human breast cancer tissue, human spleen tissue

Validation Images & Assay Conditions

Gene/Protein Information For HSF1 (Source: Uniprot.org, NCBI)

Gene Name

HSF1

Full Name

Heat shock factor protein 1

Weight

57260 MW

Superfamily

HSF family

Alternative Names

Heat shock factor protein 1;HSF 1;Heat shock transcription factor 1;HSTF 1;HSF1;HSTF1; HSF1 HSTF1 heat shock transcription factor 1 heat shock factor protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSF1, check out the HSF1 Infographic

HSF1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00250 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Higher heat shock factor 1 expression in tumor stroma predicts poor prognosis in esophageal squamous cell carcinoma patients

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4 Customer Q&As for Anti-Phospho-HSF1 (S326) Rabbit Monoclonal Antibody

Question

We are currently using anti-Phospho-HSF1 (S326) Rabbit Monoclonal antibody P00250 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

K. Evans

Verified customer

Asked: 2019-11-22

Answer

The anti-Phospho-HSF1 (S326) Rabbit Monoclonal antibody (P00250) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-22

Question

My colleagues were well pleased with the WB result of your anti-Phospho-HSF1 (S326) Rabbit Monoclonal antibody. However we have observed positive staining in testis nucleus using this antibody. Is that expected? Could you tell me where is HSF1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-12-14

Answer

From literature, testis does express HSF1. Generally HSF1 expresses in nucleus. Regarding which tissues have HSF1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-12-14

Question

Our lab used your anti-Phospho-HSF1 (S326) Rabbit Monoclonal antibody for IHC on testis last year. I am using human, and We are going to use the antibody for WB next. I was wanting to use examining testis as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for WB?

M. Baker

Verified customer

Asked: 2017-05-25

Answer

I have checked the website and datasheets of our anti-Phospho-HSF1 (S326) Rabbit Monoclonal antibody and I see that P00250 has been tested on human in both IHC and WB. Thus P00250 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-05-25

Question

We have observed staining in human muscle. Any tips? Is anti-Phospho-HSF1 (S326) Rabbit Monoclonal antibody supposed to stain muscle positively?

M. Krishna

Verified customer

Asked: 2013-01-23

Answer

From what I have seen in literature muscle does express HSF1. From what I have seen in Uniprot.org, HSF1 is expressed in testis, muscle, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have HSF1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-01-23

Order DetailsPrice
P00250

100μL

$370
P00250-30ul

30μL sample

$99
P00250-carrier-free-1ml

Carrier Free

$2590

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