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Product Info Summary
| SKU: | A00268Y896 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Phospho-IRS-1 (Y896) Antibody
SKU/Catalog Number
A00268Y896
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-IRS-1 (Y896) Antibody catalog # A00268Y896. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-IRS-1 (Y896) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00268Y896)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Tyr896. AA range:862-911
Reactive Species
A00268Y896 is reactive to IRS1 in Human, Mouse, Rat
Calculated molecular weight
131.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00268Y896 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Western blot analysis of KB MCF-7 using p-IRS-1 (Y896) antibody. Antibody was diluted at 1:500
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Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using IRS-1 (Phospho-Tyr896) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Phospho-IRS-1 (Y896) Antibody (A00268Y896)
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