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Product Info Summary
| SKU: | PB9391 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SHC/SHC1 Antibody Picoband®
SKU/Catalog Number
PB9391
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SHC/SHC1 Antibody Picoband® catalog # PB9391. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHC/SHC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9391)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SHC, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9391 is reactive to SHC1 in Human, Mouse, Rat
Observed Molecular Weight
63 kDa
Calculated molecular weight
62.8 kDa
Background of SHC1
SHC, also known as SHC1 (SHC-transforming protein 1) or SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9391 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human Hela whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of SHC1 using anti-SHC1 antibody (PB9391).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHC1 antigen affinity purified polyclonal antibody (PB9391) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SHC1 at approximately 63 kDa. The expected band size for SHC1 is at 63 kDa.
Specific Publications For Anti-SHC/SHC1 Antibody Picoband® (PB9391)
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2 Customer Q&As for Anti-SHC/SHC1 Antibody Picoband®
Question
We are currently using anti-SHC/SHC1 antibody PB9391 for mouse tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
A. Kulkarni
Verified customer
Asked: 2019-04-25
Answer
The anti-SHC/SHC1 antibody (PB9391) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-25
Question
I am interested in your PB9391 and PA1897 SHC1 antibody, have you also saw 3 isoforms in any of your tested samples?
Verified Customer
Verified customer
Asked: 2017-09-13
Answer
Please see the updated image of PB9391 and PA1897 on the product pages. https://www.bosterbio.com/anti-shc-picoband-trade-antibody-pb9391-boster.html?options=cart https://www.bosterbio.com/anti-shc-antibody-pa1897-boster.html?options=cart Both PB9391 and PA1897 are able to detect 3 isoforms.
Boster Scientific Support
Answered: 2017-09-13
