Product Info Summary
SKU: | A02608Y185-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody
SKU/Catalog Number
A02608Y185-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody catalog # A02608Y185-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02608Y185-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human JNK1/2/3 around the phosphorylation site of Y185.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02608Y185-1 is reactive to MAPK8 in Human, Mouse, Rat
Applications
A02608Y185-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48.296kDa
Background of JNK1
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.
Tsai L.-H., Nature 371:419-423(1994).
Patrick G.N., Nature 402:615-622(1999).
Kesavapany S., J. Neurosci. 24:4421-4431(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
Validation Images & Assay Conditions
Click image to see more details
Figure 10. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of mouse brain tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 11. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of rat brain tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 12. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of rat kidney tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 13. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of rat liver tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 14. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of rat lung tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 15. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of rat spinal cord tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 16. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of rat testis tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 2. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human-appendix tissue. Anti-Phospho-JNK1/2/3 (Y185) at 1:200 (4°CClick image to see more details
Figure 3. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human colon tissue. Anti-Phospho-JNK1/2/3 (Y185) at 1:200 (4°CClick image to see more details
Figure 4. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human liver cancer tissue. Anti-Phospho-JNK1/2/3 (Y185) at 1:200 (4°CClick image to see more details
Figure 5. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human lung cancer tissue. Anti-Phospho-JNK1/2/3 (Y185) at 1:200 (4°CClick image to see more details
Figure 6. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human lung tissue. Anti-Phospho-JNK1/2/3 (Y185) at 1:200 (4°CClick image to see more details
Figure 7. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human stomach cancer tissue. Anti-Phospho-JNK1/2/3 (Y185) at 1:200 (4°CClick image to see more details
Figure 8. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human uterus cancer tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 9. Immunohistochemistry validation of MAPK8 using Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1).
Immunohistochemical analysis of human uterus tissue. A02608Y185-1 was diluted at 1:200 (4°CClick image to see more details
Figure 1. Western blotting validation for Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody A02608Y185-1
Western blot (WB) analysis of JNK1/2/3 (phospho Tyr185) polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MAPK8 (Source: Uniprot.org, NCBI)
Gene Name
MAPK8
Full Name
Mitogen-activated protein kinase 8
Weight
48.296kDa
Superfamily
protein kinase superfamily
Alternative Names
c-Jun N-terminal kinase 1; EC 2.7.11; EC 2.7.11.24; JNK1 alpha protein kinase; JNK1 beta protein kinase; JNK1; JNK1JNK1A2; JNK-46; JUN N-terminal kinase; MAP kinase 8; MAPK 8; MAPK8; mitogen-activated protein kinase 8 isoform JNK1 alpha1; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8; PRKM8; PRKM8JNK; protein kinase JNK1; SAPK1; SAPK1JNK21B1/2; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1 MAPK8 JNK, JNK-46, JNK1, JNK1A2, JNK21B1/2, PRKM8, SAPK1, SAPK1c mitogen-activated protein kinase 8 mitogen-activated protein kinase 8|JUN N-terminal kinase|MAP kinase 8|c-Jun N-terminal kinase 1|stress-activated protein kinase 1|stress-activated protein kinase 1c
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAPK8, check out the MAPK8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAPK8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-JNK1/2/3 (Y185) MAPK8 Antibody (A02608Y185-1)
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