This website uses cookies to ensure you get the best experience on our website.
- Table of Content
(NOT SET)
Human ICAM-1 / CD54 ELISA Kit PicoKine™
Human ICAM-1 / CD54 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. Cited in 19 publication(s).
Human ICAM-1 / CD54 ELISA Kit PicoKine™ Product Summary
| SKU: | EK0370 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10 pg/ml |
| Assay Range: | 156 pg/ml - 10,000 pg/ml |
Overview
| Product Name | Human ICAM-1 / CD54 ELISA Kit PicoKine™
See more ICAM-1/CD54 products |
|---|---|
| Catalog# | EK0370 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) |
| Description | Human ICAM-1 / CD54 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product | Human ICAM-1 / CD54 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0370) |
| ELISA Validation | ELISA Validation Information |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10 pg/ml |
| Assay Range | 156 pg/ml - 10,000 pg/ml |
| Immunogen/Standard | Expression system for standard: NS0; Immunogen sequence: Q28-E480 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96 wells/kit, with removable strips. |
Gene/Protein Basic Information For ICAM1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P05362 |
|---|---|
| NCBI Gene Id | 3383 |
| Species Of This Entry | Human |
| Gene Name | ICAM1 |
| Protein Name | Intercellular adhesion molecule 1 |
| Superfamily | immunoglobulin superfamily |
| Alternative Names | ICAM-1/CD54|BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 |
| Molecular Weight | 57825 |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ICAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Kit Components And Assay QC Data Details Of Human ICAM-1 / CD54 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0370-CAP | Anti-Human ICAM1 Pre-coated 96-well strip microplate | 1 |
| EK0370-ST | Human ICAM1 Standard | 2 vials, 10 ng/tube |
| EK0370-DA | Human ICAM1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0370 Human ICAM-1 / CD54 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.001 | 0.058 | 0.106 | 0.208 | 0.526 | 1.025 | 1.503 | 2.098 |
We measured random samples of Human ICAM-1 / CD54 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 272 | 1923 | 4746 | 248 | 1929 | 4549 |
| Standard deviation | 14.41 | 111.53 | 204.07 | 14.13 | 125.38 | 236.54 |
| CV (%) | 5.3% | 5.8% | 4.3% | 5.7% | 6.5% | 5.2% |
We measured the performance consistency of Human ICAM-1 / CD54 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 272 | 260 | 271 | 261 | 266 | 5.52 | 2% |
| Sample 2 | 1923 | 1695 | 1731 | 1759 | 1777 | 87.29 | 4.9% |
| Sample 3 | 4746 | 4742 | 4337 | 4571 | 4599 | 166.94 | 3.6% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 19
| Gökçay Canpolat A,Cinel M,Dizbay Sak S,Taşkaldıran I,Korkmaz H,Demir Ö,Ersoy R,Dağdelen S,Berker D,Dalva K,Bahçecioğlu Mutlu A, B,Erdoğan M, F:Long-Term Outcomes of Tamoxifen Citrate Therapy and Histo- and Immunopathological Properties in Riedel Thyroidit Species: Human EK0370 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA |
View Publication |
| Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial | PubMed ID 27651815 |
| Comparison of the effects of Crataegus oxyacantha extract, aerobic exercise and their combination on the serum levels of ICAM-1 and E-Selectin in patients with stable angina pectoris | PubMed ID 26687477 |
| Neurally mediated syncope: Is it really an endothelial dysfunction? | PubMed ID 26680551 |
| Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults | PubMed ID 26664425 |
| FGF-23/Vitamin D Axis in Type 1 Diabetes: The Potential Role of Mineral Metabolism in Arterial Stiffness | PubMed ID 26462160 |
| Maggi P, Bruno G, Perilli F, Saracino A, Volpe A, Santoro C, Ladisa N, Angarano G. In Vivo. 2017 Jan 2;31(1):125-131. Effects of Therapy with Maraviroc on the Carotid Intima Media Thickness in HIV-1/HCV Co-infected Patients | PubMed ID 28064231 |
| Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals. | PubMed ID 24575134 |
| Lu Y, Zhu X, Liang Gx, Cui Rr, Liu Y, Wu Ss, Liang Qh, Liu Gy, Jiang Y, Liao Xb, Xie H, Zhou Hd, Wu Xp, Yuan Lq, Liao Ey. Amino Acids. 2012 Nov;43(5):2125-36. Doi: 10.1007/S00726-012-1298-7. Epub 2012 Apr 25. Apelin-Apj Induces Icam-1, Vcam-1 And ... | PubMed ID 22532031 |
| Xiang M, Qian Zy, Zhou Ch, Liu J, Li Wn. J Ethnopharmacol. 2006 Aug 11;107(1):25-31. Epub 2006 Mar 10. Crocetin Inhibits Leukocyte Adherence To Vascular Endothelial Cells Induced By Ages. | PubMed ID 16529888 |
| Tian Xx, Wang Bl, Cao Yz, Zhong Yx, Tu Yy, Xiao Jb, He Qf, Zhai Ln. Chin J Integr Med. 2015 Mar;21(3):229-33. Doi: 10.1007/S11655-013-1513-8. Epub 2013 Oct 19. Comparison Of Protective Effects Of Safflor Injection And Extract Of Ginkgo Biloba On L... | PubMed ID 24142260 |
| Yang Py, Rui Yc, Lu L, Li Tj, Liu Sq, Yan Hx, Wang Hy. Life Sci. 2005 Sep 30;77(20):2529-39. Time Courses Of Vascular Endothelial Growth Factor And Intercellular Adhesion Molecule-1 Expressions In Aortas Of Atherosclerotic Rats. | PubMed ID 16005023 |
| Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L.... | PubMed ID 23625305 |
| Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients? | PubMed ID 25865191 |
| Jiang Jx, Zhang Sj, Liu Yn, Lin Xx, Sun Yh, Shen Hj, Yan Xf, Xie Qm. Eur J Pharmacol. 2014 Mar 15;727:43-51. Doi: 10.1016/J.Ejphar.2014.01.045. Epub 2014 Jan 31. Eets Alleviate Ox-Ldl-Induced Inflammation By Inhibiting Lox-1 Receptor Expression In... | PubMed ID 24486707 |
| Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells. | PubMed ID 24333549 |
| Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M... | PubMed ID 23219582 |
| Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce... | PubMed ID 21647942 |
| Jing L, Wang Jg, Zhang Jz, Cao Cx, Chang Y, Dong Jd, Guo Fy, Li Pa. J Inflamm (Lond). 2014 Nov 5;11(1):35. Doi: 10.1186/S12950-014-0035-2. Ecollection 2014. Upregulation Of Icam-1 In Diabetic Rats After Transient Forebrain Ischemia And Reperfusion... | PubMed ID 25389378 |
Submit your review to [email protected] or to Biocompare.com for an Amazon giftcard.
0 reviews
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0370 Human ICAM-1 / CD54 ELISA Kit PicoKine™
8 Related Questions
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2020-10-16
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-10-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Y. Green
Asked: 2020-02-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-11
Question
Q: how do I analyze ELISA data? I have performed CD54 level in serum.
Verified Customer
Asked: 2019-10-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-10-10
Question
Q: if the enzyme conjugated CD54 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD54 antigen?
Verified Customer
Asked: 2019-04-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-12
Question
Q: is it okay to use citrate plasma as samples in Human CD54 Picokine® ELISA Kit (Catalog # EK0370)?
Verified Customer
Asked: 2019-02-03
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD54 causing disruption of its protein structure. CD54 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD54 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-02-03
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Asked: 2018-01-30
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-01-30
Question
Q: how can I thaw whole blood sample for CD54 ELISA after freezing?
Verified Customer
Asked: 2016-02-15
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD54 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-02-15
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD54 using the EK0370 Human CD54 Picokine® ELISA Kit?
H. Rodriguez
Asked: 2016-01-07
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2016-01-07
Order Product (EK0370)
Shipping Information
Domestically in USA and Canada, ships next business day if available in stock.
Contact Information
| [email protected] | |
| (888) 466-3604 |
Boster Guarantee
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.
See Details
In stock
Order Product
EK0370
$369.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.