Mouse ICAM-1 / CD54 ELISA Kit PicoKine™

Mouse ICAM-1 / CD54 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Icam1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 19 publication(s).

Product Info Summary

SKU: EK0371
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Mouse ICAM-1 / CD54 ELISA Kit PicoKine™

See all ICAM-1/CD54 products

SKU/Catalog Number

EK0371

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse ICAM-1 / CD54 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Icam1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse ICAM-1 / CD54 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0371)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q28-N485

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0371 is reactive to Icam1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK0371 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0371-CAP Anti-Mouse Icam1 Pre-coated 96-well strip microplate 1
EK0371-ST Mouse Icam1 Standard 2 vials, 10 ng/tube
EK0371-DA Mouse Icam1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0180.0540.1050.1890.4580.7591.4682.267

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse ICAM-1 / CD54 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3541378497836114585187
Standard deviation21.9462.01383.1926.2%783.1414.96
CV (%)6.2%4.5%6.4%7%5.7%8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ICAM-1/CD54 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135436235733435110.633%
Sample 213781386135316041430101.057%
Sample 34978475249114804486188.481.8%
*number of samples for each test n=16.

Gene/Protein Information For Icam1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P13597

Gene ID

15894

Gene Name

Icam1

Full Name

Intercellular adhesion molecule 1

Weight

58.844kDa

Superfamily

immunoglobulin superfamily

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 Icam1|CD54, Icam, Icam-1, Ly-4, Ly-47, MAL, MALA-2|intercellular adhesion molecule 1|intercellular adhesion molecule 1|myD10

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Icam1, check out the Icam1 Infographic

Icam1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Icam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0371 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Guo J,Li M,Yang Y,Zhang L,Zhang LW,Sun QY.Pretreatment with atorvastatin ameliorates cobra venom factor-induced acute lung inflammation in mice.BMC Pulm Med.2020 Oct 12;20(1):263.doi:10.1186/s12890-020-01307-3.PMID:33046059;PMCID:PMC7552367.
Species: Mouse
EK0371 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0371 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

The role of superoxide dismutase in the survival of Mycobacterium tuberculosis in macrophages

Anti-psoriatic effects of Honokiol through the inhibition of NF-κB and VEGFR-2 in animal model of K14-VEGF transgenic mouse

Aldosterone induced up-expression of ICAM-1 and ET-1 in pancreatic islet endothelium may associate with progression of T2D.

Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

Urocortin promotes the development of vasculitis in a rat model of thromboangiitis obliterans via corticotrophin-releasing factor type 1 receptors

Xin J, Sun H, Kong H, Li L, Zheng J, Yin C, Cao Y, Jia Y, Li C. Mol Med Rep. 2015 May;11(5):3391-7. Doi: 10.3892/Mmr.2015.3174. Epub 2015 Jan 9. Intercellular Adhesion Molecule-1 Expression In Activated Eosinophils Is Associated With Mucosal Remod...

Xiang M, Qian Zy, Zhou Ch, Liu J, Li Wn. J Ethnopharmacol. 2006 Aug 11;107(1):25-31. Epub 2006 Mar 10. Crocetin Inhibits Leukocyte Adherence To Vascular Endothelial Cells Induced By Ages.

Tian Xx, Wang Bl, Cao Yz, Zhong Yx, Tu Yy, Xiao Jb, He Qf, Zhai Ln. Chin J Integr Med. 2015 Mar;21(3):229-33. Doi: 10.1007/S11655-013-1513-8. Epub 2013 Oct 19. Comparison Of Protective Effects Of Safflor Injection And Extract Of Ginkgo Biloba On L...

Yang Py, Rui Yc, Lu L, Li Tj, Liu Sq, Yan Hx, Wang Hy. Life Sci. 2005 Sep 30;77(20):2529-39. Time Courses Of Vascular Endothelial Growth Factor And Intercellular Adhesion Molecule-1 Expressions In Aortas Of Atherosclerotic Rats.

Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L....

Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients?

Jiang Jx, Zhang Sj, Liu Yn, Lin Xx, Sun Yh, Shen Hj, Yan Xf, Xie Qm. Eur J Pharmacol. 2014 Mar 15;727:43-51. Doi: 10.1016/J.Ejphar.2014.01.045. Epub 2014 Jan 31. Eets Alleviate Ox-Ldl-Induced Inflammation By Inhibiting Lox-1 Receptor Expression In...

Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.

Feng L, Zhu M, Zhang M, Jia X, Cheng X, Ding S, Zhu Q. Int Immunopharmacol. 2013 Feb;15(2):206-16. Doi: 10.1016/J.Intimp.2012.11.015. Epub 2012 Dec 5. Amelioration Of Compound 4,4'-Diphenylmethane-Bis(Methyl)Carbamate On High Mobility Group Box1-M...

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

Jing L, Wang Jg, Zhang Jz, Cao Cx, Chang Y, Dong Jd, Guo Fy, Li Pa. J Inflamm (Lond). 2014 Nov 5;11(1):35. Doi: 10.1186/S12950-014-0035-2. Ecollection 2014. Upregulation Of Icam-1 In Diabetic Rats After Transient Forebrain Ischemia And Reperfusion...

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6 Customer Q&As for Mouse ICAM-1 / CD54 ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I have obtained CD54 level in serum.

L. Wang

Verified customer

Asked: 2020-07-17

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-07-17

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-05-26

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-26

Question

Q: What is the optimal O.D. value for CD54 ELISA kit? I used your CD54 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD54 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-02-15

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD54 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD54 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-02-15

Question

Q: if the enzyme conjugated CD54 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD54 antigen?

V. Singh

Verified customer

Asked: 2019-06-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-06-30

Question

Q: how can I thaw whole blood sample for CD54 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-09-29

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD54 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-09-29

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-09-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-09-28

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