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- Table of Contents
POLR2A antibody (P01029-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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Immunofluorescent analysis of MCF7 cells, using Phospho-POLR2A (S2) Antibody .
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Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody
POLR2A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
POLR2A antibody was positively detected by WB in:
human MCF-7 whole cell, human SH-SY5Y whole cell, human SIHA whole cell, human U251 whole cell, rat C6 whole cell, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
POLR2A antibody was positively detected by IHC in:
human breast cancer tissue, mouse thymus tissue, rat thymus tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
POLR2A antibody was positively detected by ICC/IF in:
MCF7 cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody
View all POLR2A Antibodies
View all POLR2A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human POLR2A
Observed Molecular Weight
270 kDa
Calculated Molecular Weight
217.2 kDa
Cite This Product
Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01029-1)
Protocols
Western Blot protocol for POLR2A antibody
Immunohistochemistry (IHC) protocol for POLR2A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for POLR2A antibody
Publications
Application:
Heparanase mediates vascular endothelial growth factor gene transcription in high-glucose human retinal microvascular endothelial cells.
PMID: 28848320
Review
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Product Promise
We promise Anti-Phospho-POLR2A (S2) Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
P01029-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026