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- Table of Contents
SQSTM1 antibody (PA1955) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 10 Publications
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Flow Cytometry analysis of A549 cells using anti-SQSTM1 antibody (PA1955).
Overlay histogram showing A549 cells stained with PA1955 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SQSTM1 Antibody (PA1955,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A549 cells stained with PA1955 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SQSTM1 Antibody (PA1955,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-SQSTM1/p62 Antibody Picoband®
SQSTM1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
SQSTM1 antibody was positively detected by WB in:
human Hela whole cell, human COLO-320 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SQSTM1 antibody was positively detected by IHC in:
rat brain tissue, human gastric cancer tissue, human lung cancer tissue, human oesophagus squama cancer tissue, human rectal cancer tissue, human sarcoma tissue, human cholangiocarcinoma tissue, human liver cancer tissue, mouse brain tissue, rat liver tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SQSTM1 antibody was positively detected by ICC/IF in:
A549 cell
SQSTM1 antibody was positively detected by FC in:
A549 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SQSTM1/p62 Antibody Picoband®
View all SQSTM1 Antibodies
View all SQSTM1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1, different from the related rat and mouse sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
60 kDa
Calculated Molecular Weight
47.7 kDa
Background of SQSTM1
SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
Cite This Product
Anti-SQSTM1/p62 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1955)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
95%
High Homology
Rat
95%
High Homology
Cow
95%
High Homology
Sheep
95%
High Homology
Zebrafish
55%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SQSTM1 antibody
Immunohistochemistry (IHC) protocol for SQSTM1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SQSTM1 antibody
Flow Cytometry (FC) protocol for SQSTM1 antibody
Publications
Application:
Species:
Liu, J.,Qin, X.,Ma, W.,Jia, S.,Zhang, X.,Yang, X. ... Jin, F. (2021). Corilagin induces apoptosis and autophagy in NRF2‑addicted U251 glioma cell line. Molecular Medicine Reports,23,320. https://doi.org/10.3892/mmr.2021.11959
Species: Human
PMID: -
Zhong W,Tang X,Liu Y,Zhou C,Liu P,Li E,Zhong P,Lv H,Zou Q,Wang M.Benzoxazole Derivative K313 Induces Cell Cycle Arrest, Apoptosis and Autophagy Blockage and Suppresses mTOR/p70S6K Pathway in Nalm-6 and Daudi Cells.Molecules.2020 Feb 21;25(4):971.doi:10.33
Species: Human
PMID: 32098126
Liu X,Feng C,Wei G,Kong W,Meng H,Du Y,Li J.Mitofusin1 Is a Major Mediator in Glucose-Induced Epithelial-to-Mesenchymal Transition in Lung Adenocarcinoma Cells.Onco Targets Ther.2020 Apr 24;13:3511-3523.doi:10.2147/OTT.S238714.PMID:32425551;PMCID:PMC718794
Species: Human
PMID: 32425551
Yuan H,Wang Y,Chen H,Cai X.Protective effect of flavonoids from Rosa roxburghii Tratt on myocardial cells via autophagy.3 Biotech.2020 Feb;10(2):58.doi:10.1007/s13205-019-2049-1.Epub 2020 Jan 22.PMID: 32015954;PMCID:PMC6976074.
Species: Rat
PMID: 32015954
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
PMID: 31900522
Regulation of autophagy by AMP-activated protein kinase/sirtuin 1 pathway reduces spinal cord neurons damage
PMID: 29085598
Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice
PMID: 28266555
Nrf2 signalling andautophagyare involved in diabetes mellitus-induced defects in the development of mouse placenta
PMID: 27383629
Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock?19/+ mice contributes to improved glucose homeostasis
PMID: 26228022
Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis
PMID: 26120766
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Product Promise
We promise Anti-SQSTM1/p62 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1955
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026