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Product Info Summary
| SKU: | P00007-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00007-1
BM4169 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody catalog # P00007-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00007-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CAA-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-STAT3 (S727)
Reactive Species
P00007-1 is reactive to STAT3 in Human, Mouse, Rat
Observed Molecular Weight
88 kDa
Calculated molecular weight
88.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00007-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
IHC: rat brain tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of STAT3 using anti-STAT3 antibody (P00007-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human U251 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified monoclonal antibody (Catalog # P00007-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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IF analysis of STAT3 using anti-STAT3 antibody (P00007-1).
STAT3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated at 1:50 with rabbit anti-STAT3 Antibody (P00007-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rat brain, using Phospho-STAT3 (S727) Antibody.
Specific Publications For Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody (P00007-1)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody P00007-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody (P00007-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
My boss were well pleased with the WB result of your anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody. However we have been able to see positive staining in kidney pancreas cytoplasm. nucleus using this antibody. Is that expected? Could you tell me where is STAT3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
From literature, kidney pancreas does express STAT3. Generally STAT3 expresses in cytoplasm. nucleus. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2019-12-09
Question
We purchased anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody for IP on cervix carcinoma last year. I am using rat, and We intend to use the antibody for IF next. We want examining cervix carcinoma as well as upper lobe of lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
D. Mitchell
Verified customer
Asked: 2018-12-11
Answer
I looked at the website and datasheets of our anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody and it seems that P00007-1 has been tested on rat in both IP and IF. Thus P00007-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-11
Question
you antibody using your anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody for response to peptide hormone studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
C. Jones
Verified customer
Asked: 2016-11-28
Answer
I appreciate your inquiry. This P00007-1 anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for IP, IF, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-11-28
Question
We have been able to see staining in human upper lobe of lung. Are there any suggestions? Is anti-Phospho-STAT3 (S727) Rabbit Monoclonal antibody supposed to stain upper lobe of lung positively?
D. Moore
Verified customer
Asked: 2013-11-11
Answer
From literature upper lobe of lung does express STAT3. From Uniprot.org, STAT3 is expressed in upper lobe of lung, placenta, kidney pancreas, liver, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have STAT3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Erythroleukemia, Pubmed ID: 23186163
Kidney, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 7701321, 24275569
Placenta, Pubmed ID: 7512451
Boster Scientific Support
Answered: 2013-11-11
