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PIK3R1 antibody (A00318-1) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 26 Publications

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a00318 1 pik3 primary antibodies fcm testing 8

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Flow Cytometry analysis of HL-60 cells using anti-PI 3 Kinase p85 alpha/PIK3R1 antibody (A00318-1).
Overlay histogram showing HL-60 cells stained with A00318-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody (A00318-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®

Name: Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Brand: Boster Bio
SKU: A00318-1
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

PIK3R1 antibody

26 Publications|

2 Reviews

|Submit a Review

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, Flow Cytometry, IF, IHC, ICC, WB

Tested Applications

PIK3R1 antibody was positively detected by WB in:

human Jurkat whole cell, human Hela whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

PIK3R1 antibody was positively detected by IHC in:

human lung cancer tissue, human lymphoma tissue, human testicular cancer tissue, mouse brain tissue, rat brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

PIK3R1 antibody was positively detected by ICC/IF in:

Caco-2 cell

PIK3R1 antibody was positively detected by FC in:

HL-60 cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Bovine

Predicted

Monkey

Predicted

Rabbit

Predicted

Dog

Predicted

Product Information

Product Name

Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
View all PIK3R1 Antibodies

Form

Lyophilized

Uniprot

P27986

Gene ID

5295

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PI 3 Kinase p85 alpha/PIK3R1 recombinant protein (Position: D117-Q153).

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

85 kDa

Calculated Molecular Weight

83.6 kDa

Background of PIK3R1

Phosphatidylinositol 3-kinase regulatory subunit alpha is an enzyme that in humans is encoded by the PIK3R1 gene. Its gene is mapped to 5q13. the bovine PI3K p85 subunit consists of 2 closely related proteins, p85-alpha and p85-beta. They cloned cDNAs encoding both p85 subunits, each of which is a 724-amino acid polypeptide. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.

Cite This Product

Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00318-1)

Protocols

Western Blot protocol for PIK3R1 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for PIK3R1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PIK3R1 antibody

Review Protocol

Flow Cytometry (FC) protocol for PIK3R1 antibody

Review Protocol

Publications

16α-Hydroxydehydrotrametenolic acid attenuates MDA-MB-231 triple-negative breast cancer xenograft growth and modulates integrin β1/c-Met signaling and glycolytic metabolism

Published: 2026-08-19

Journal: PHYTOMEDICINE

Application: WB

Impact Factor: 11.30

Species: Human

PMID: 42641546

SKU	A00318-1
Application	Western Blot
Sample	Mouse brain tissue
Sample Processing Description	Mouse brain tissue was lysed in RIPA buffer supplemented with protease inhibitors at 4°C for 2 hours. The lysate was centrifuged to collect the supernatant, and protein concentration was determined. After adjusting the concentration, samples were mixed with 5× protein loading buffer and heated at 95–100°C for 10 minutes to denature. Then, 15 μL of each protein sample was loaded per lane for electrophoresis.
Other Reagents	Blocking buffer
Primary Antibody	PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®
Primary Incubation	1:2000, overnight at 4 ℃
Secondary Antibody	HRP Goat Anti-Rabbit IgG
Secondary Incubation	1:10000, 1 hour in room temperature
Detection	Substrate: ECL, Imaging system:ChemiDoc MP
Results Summary	The figure shows a schematic representation of Western blot results for PIK3R1 and the loading control β-actin in mouse brain tissues from normal mice, model group, and mice treated with high and low doses of drug AB. Although the expression differences between experimental groups are not pronounced, the Western blot results obtained with this antibody are still clear and well-defined.

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Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®

PIK3R1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

16α-Hydroxydehydrotrametenolic acid attenuates MDA-MB-231 triple-negative breast cancer xenograft growth and modulates integrin β1/c-Met signaling and glycolytic metabolism

Rhodiosin promotes cerebral angiogenesis in mice with ischemic stroke via PI3K/Akt pathway activation

A Pathological Microenvironment–Responsive Catalytic Carbon Nanodots Release Platform Intercepting the ROS–Inflammation Cascade to Alleviate Intervertebral Disc Degeneration

S-equol promotes ferroptosis in triple negative breast cancer by coordinating NCOA4-mediated ferritinophagy and PPARγ-mediated lipid metabolism

BEZ235 enhances the photodynamic antitumor efficacy of hypoxia-responsive BODIPY prodrug nanoparticles by inhibiting the PI3K/mTOR pathway and alleviating hypoxia

Transcriptomics and metabolomics reveal functional nanoplastics-induced male reproductive damage and resveratrol antagonistic effects

Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis

ApoE-T807-modified tanshinone IIA liposomes enhance cognitive function in APP/PS1 mice by inhibiting Tau phosphorylation via the PI3K/AKT signaling pathway

Therapeutic mechanism of Jinshui Liujunzi Decoction in treating COPD: targeting the PI3K/Akt signaling pathway

The Therapeutic Mechanisms of Shenyan Oral Liquid I Against Chronic Kidney Disease Based on Network Pharmacology and Experimental Validation

Sappanone A attenuates rheumatoid arthritis via inhibiting PI3K/AKT/NF-κB and JAK2/STAT3 signaling pathways in vivo and in vitro

Sinomenine Alleviates Rheumatoid Arthritis by Suppressing the PI3K-Akt Signaling Pathway, as Demonstrated Through Network Pharmacology, Molecular Docking, and Experimental Validation

The role of phosphoprotein associated with glycosphingolipid-enriched microdomains 1 (PAG1) in regulating the progression of oral squamous cell carcinoma

Mechanism investigation of Duhuo Jisheng pill against rheumatoid arthritis based on a strategy for the integration of network pharmacology, molecular docking and in vivo experimental verification

Effects of Cooling Interventions with Different Target Temperatures on Heat Stroke Rats

Triptolide inhibits the Apoptosis and Reactive Oxygen Species Production in Hydrogen Peroxide-Treated Nasal Epithelial Cells by Regulating PTEN/PI3K/AKT Signaling Pathway

Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway

Effect and mechanism of PI3K/AKT/mTOR signaling pathway in the apoptosis of GC-1 cells induced by nickel nanoparticles

Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway

Hypoglycemic Mechanism of the Berberine Organic Acid Salt under the Synergistic Effect of Intestinal Flora and Oxidative Stress

Kallikrein-related peptidase 4 contributes to the tumor metastasis of oral squamous cell carcinoma

SPOCK1 promotes the proliferation, migration and invasion of glioma cells through PI3K/AKT and Wnt/β-catenin signaling pathways

Suppressive oligodeoxynucleotides induced tolerogenic plasmacytoid dendritic cells and ameliorated the experimental autoimmune neuritis

Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells

Resveratrol attenuates intermittent hypoxia‑induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol‑4,5‑bisphosphate 3‑kinase/AKT pathway

Wogonin induces apoptosis and down-regulates survivin in human breast cancer MCF-7 cells by modulating PI3K–AKT pathway

Review

Have you used Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®?

2 Reviews For Anti-PI 3 Kinase p85 alpha/PIK3R1 Antibody Picoband®

This antibody is highly specific and efficient, suitable for Western blot detection of Anti-PI3K p85 alpha Antibody protein in mouse hippocampus, with only minor nonspecific bands observed.

The antibody shows clear bands with no non-specific signals in WB, and is suitable for detecting PIK3R1 protein in mouse hippocampus by Western blot.

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