Rabbit IgG polyclonal antibody for Osteopontin(SPP1) detection. Tested with WB, IHC-P in Human. Cited in 5 publication(s).
Anti-Osteopontin/SPP1 Antibody Info At A Glance
|Product Name||Anti-Osteopontin/SPP1 Antibody
See all SPP1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Osteopontin/SPP1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Osteopontin/SPP1 information: Molecular Weight: 35423 MW; Subcellular Localization: Secreted; Tissue Specificity: Bone. Found in plasma.|
|Cite This Product||Anti-Osteopontin/SPP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1431)|
|Similar Products From
|Anti-Osteopontin/SPP1 Antibody may replace the following items: sc 167294.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human Osteopontin(28-47aa EEKQLYNKYPDAVATWLNPD), different from the related mouse sequence by six amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-Osteopontin/SPP1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: MM231 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: COLO320 Cell Lysate
IHC(P): Human Mammary Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 5
|Shan A, Zhou C, He Y, Feng W, Chen G, Zhong G. Ren Fail. 2013;35(1):37-42. Doi: 10.3109/0886022X.2012.741645. Epub 2012 Nov 14. Expression Of Both Matrix Metalloproteinase-2 And Its Tissue Inhibitor-2 In Tunica Media Of Radial Artery In Uremic Pat...||PubMed ID 23151010|
|Aksoy A, Artas G, Sevindik OG. Pak J Med Sci. 2017 May-Jun;33(3):560-565. doi: 10.12669/pjms.333.12559. Predictive value of stathmin-1 and osteopontin expression for taxan resistance in metastatic castrate-resistant prostate cancer||PubMed ID 28811771|
|Effects of Naringin on Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells In Vitro and In Vivo||PubMed ID 26078764|
|Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension||PubMed ID 27391973|
|(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor||PubMed ID 29218094|
Order Product (PA1431)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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