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- Table of Contents
PIK3CB antibody (M01091-1) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IHC analysis of PI3 Kinase p110 Beta/PIK3CB using anti-PI3 Kinase p110 Beta/PIK3CB antibody (M01091-1).
PI3 Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PI3 Kinase p110 Beta/PIK3CB Antibody (M01091-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
PI3 Kinase p110 Beta/PIK3CB was detected in a paraffin-embedded section of human colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1: 50 rabbit anti-PI3 Kinase p110 Beta/PIK3CB Antibody (M01091-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-PI3 Kinase p110 beta Rabbit Monoclonal Antibody
PIK3CB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
PIK3CB antibody was positively detected by IHC in:
human colon tissue, human colon cancer tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
Human
Recommend
Tested
Recommend
Recommend
Mouse
Recommend
Tested
Recommend
Recommend
Rat
Recommend
Tested
Recommend
Recommend
Product Information
Product Name
Anti-PI3 Kinase p110 beta Rabbit Monoclonal Antibody
View all PIK3CB Antibodies
View all PIK3CB Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
30P53
Isotype
IgG
Immunogen
A synthesized peptide derived from human PI3 Kinase p110 beta
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
123 kDa
Calculated Molecular Weight
122.8 kDa
Cite This Product
Anti-PI3 Kinase p110 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01091-1)
Protocols
Immunohistochemistry (IHC) protocol for PIK3CB antibody
Publications
Application:
Species:
Luteolin alleviates right ventricular hypertrophy in high altitude pulmonary hypertension rats by regulating PI3K/AKT/mTOR signalling pathway
Published: 2025-07-01
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Rat
PMID: 40609617
Bioactive compound combinations from Rhodiola tangutica alleviate pulmonary vascular remodeling in high-altitude pulmonary hypertension rats through the PI3K–AKT pathway
Published: 2025-05-02
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.80
Species: Rat
PMID: 40385484
Avβ3 single-stranded DNA aptamer attenuates vascular restenosis via Ras-PI3K/MAPK pathway in rats after percutaneous transluminal coronary angioplasty
Published: 2019-12-26
Journal: ARTIFICIAL ORGANS
Application: WB
Impact Factor: 2.38
Species: Rat
PMID: 31879964
Comparative profiling of chondrogenic differentiation of mesenchymal stem cells (MSCs) driven by two different growth factors
Published: 2019-05-08
Journal: CELL BIOCHEMISTRY AND FUNCTION
Application: IF
Impact Factor: 2.14
Species: Rabbit
PMID: 31066473
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model
Published: 2015-08-15
Journal: International Journal of Clinical and Experimental Medicine
Application: WB
Impact Factor: 1.28
Species: Rat
PMID: 26550236
Review
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Product Promise
We promise Anti-PI3 Kinase p110 beta Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01091-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 11, 2026