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Product Info Summary
| SKU: | M05087 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-ACSL5 Mouse Monoclonal Antibody [Clone ID: OTI1C6]
SKU/Catalog Number
M05087
Size
100 µl
Description
ACSL5 (long-chain-fatty-acid–CoA ligase 5) activates long-chain fatty acids to acyl-CoAs, feeding both lipid biosynthesis and mitochondrial beta-oxidation; this is represented within curated fatty acyl-CoA biosynthesis frameworks. Assay context: Human/Mouse-reactive monoclonal antibody tested in WB for protein detection; frequently contextualized with secreted lipid-regulatory factors such as ANGPTL3 when connecting circulating lipid cues to intracellular fatty-acid utilization programs (putative co-panel).
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ACSL5 Mouse Monoclonal Antibody [Clone ID: OTI1C6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05087)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1C6
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 104-620 of human ACSL5 (NP_976313) produced in E.coli.
Reactive Species
M05087 is reactive to ACSL5 in Human, Mouse
Calculated molecular weight
76.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05087 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACSL5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACSL5.
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Western blot analysis of extracts (35ug) from mouse brain tissue lysate by using anti-ACSL5 monoclonal antibody (1:500).
Specific Publications For Anti-ACSL5 Mouse Monoclonal Antibody [Clone ID: OTI1C6] (M05087)
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