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Product Info Summary
| SKU: | M01091 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-PI3 Kinase p110 beta PIK3CB Rabbit Monoclonal Antibody
SKU/Catalog Number
M01091
BM4459 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PI3 Kinase p110 beta PIK3CB Rabbit Monoclonal Antibody catalog # M01091. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PI3 Kinase p110 beta PIK3CB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01091)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EIC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PI3 Kinase p110 beta
Reactive Species
M01091 is reactive to PIK3CB in Human
Observed Molecular Weight
110 kDa
Calculated molecular weight
122.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01091 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human Jurkat whole cell, human HepG2 whole cell, human MCF-7 whole cell, human HEL whole cell, human K562 whole cell
Validation Images & Assay Conditions
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Western blot analysis of PIK3CB using anti-PIK3CB antibody (M01091).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human HEL whole cell lysates,
Lane 6: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIK3CB antigen affinity purified monoclonal antibody (Catalog # M01091) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIK3CB at approximately 110 kDa. The expected band size for PIK3CB is at 123 kDa.
Specific Publications For Anti-PI3 Kinase p110 beta PIK3CB Rabbit Monoclonal Antibody (M01091)
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6 Customer Q&As for Anti-PI3 Kinase p110 beta PIK3CB Rabbit Monoclonal Antibody
Question
Would anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 work on zebrafish WB with amniotic fluid?
E. Kulkarni
Verified customer
Asked: 2020-04-10
Answer
Our lab technicians have not tested anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish amniotic fluid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-10
Question
I have attached the WB image, lot number and protocol we used for amniotic fluid using anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
Is this M01091 anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody reactive to the isotypes of PIK3CB?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The immunogen of M01091 anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody is A synthesized peptide derived from human PI3 Kinase p110 beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-06
Question
Would anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 work for WB with amniotic fluid?
A. Baker
Verified customer
Asked: 2018-12-20
Answer
According to the expression profile of amniotic fluid, PIK3CB is highly expressed in amniotic fluid. So, it is likely that anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 will work for WB with amniotic fluid.
Boster Scientific Support
Answered: 2018-12-20
Question
Our lab want to know about to test anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 on human amniotic fluid for research purposes, then I may be interested in using anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Parker
Verified customer
Asked: 2016-07-25
Answer
The products we sell, including anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody M01091, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-07-25
Question
Does M01091 anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Walker
Verified customer
Asked: 2015-10-20
Answer
As indicated on the product datasheet, M01091 anti-PI3 Kinase p110 beta Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-10-20
