SKU MA1085
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number PKB-175
Host Mouse
Ig Isotype Mouse IgG1
Applications WB

Overview

Product Name Anti-PKB Alpha Antibody (Monoclonal, PKB-175)
SKU/Catalog Number MA1085
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for PKB alpha, v-akt murine thymoma viral oncogene homolog 1 (AKT1) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-PKB Alpha Antibody (Monoclonal, PKB-175) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1085)
Host Mouse
Immunogen Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.

Images And Assay Conditions

/antibody/ma1085 1 WB anti pkb alpha antibody monoclonal pkb 175.jpg

Anti- PKB alpha antibody, MA1085, Western blotting
All lanes: Anti PKB alpha (MA1085) at 0.5ug/ml
Lane 1: Rat Ovary Tissue Lysate at 50ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 56KD
Observed bind size: 56KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P47196
Gene Name Akt1
Protein Name RAC-alpha serine/threonine-protein kinase
Tissue Specificity Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.
Alternative Names RAC-alpha serine/threonine-protein kinase;2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;RAC-PK-alpha;Akt1;
Subcellular Localization Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity). .
Molecular Weight 55735 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)- response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr- 117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation (By similarity). Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity (By similarity). Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53 (By similarity). .
Background PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

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Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240

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Publications

SOCS3 blocks HIF-1? expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3
Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway
Catalpol promotes cellular apoptosis in human HCT116 colorectal cancer cells via microRNA-200 and the downregulation of PI3K-Akt signaling pathway
Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT
Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice
Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line
Resveratrol attenuates intermittent hypoxia-induced insulin resistance in rats: Involvement of Sirtuin 1 and the phosphatidylinositol-4,5-bisphosphate 3-kinase/AKT pathway
Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats
Heparanase induced by advanced glycation end products (AGEs) promotes macrophage migration involving RAGE and PI3K/AKT pathway
Wang G, Li W, Lu X, Bao P, Zhao X. J Diabetes Complications. 2012 Jul-Aug;26(4):259-65. Doi: 10.1016/J.Jdiacomp.2012.04.007. Epub 2012 May 23. Luteolin Ameliorates Cardiac Failure In Type I Diabetic Cardiomyopathy.
Wang Gg, Li W, Lu Xh, Zhao X, Xu L. Croat Med J. 2013 Apr;54(2):171-9. Taurine Attenuates Oxidative Stress And Alleviates Cardiac Failure In Type I Diabetic Rats.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to akt antibody, pkb antibody, akt1 antibody, rac antibody