Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)

Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. Cited in 16 publication(s).

Product Info Summary

SKU: MA1085
Size: 100μg/vial
Reactive Species: Chicken, Human, Mouse, Rat
Host: Mouse
Application: WB

Product Name

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)

See all Akt1 products

SKU/Catalog Number

MA1085

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1085)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

PKB-175

Isotype

Mouse IgG1

Immunogen

Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1085 is reactive to Akt1 in Chicken, Human, Mouse, Rat

Applications

MA1085 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken

Validation Images & Assay Conditions

Gene/Protein Information For Akt1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P47196

Gene ID

24185

Gene Name

Akt1

Full Name

RAC-alpha serine/threonine-protein kinase

Weight

55.735kDa

Superfamily

protein kinase superfamily

Alternative Names

AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 2.7.11.1; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 Akt1|Akt|AKT serine/threonine kinase 1|RAC-alpha serine/threonine-protein kinase|AKT1 kinase|PKB alpha|RAC protein kinase alpha RAC-PK alpha|RAC-PK-alpha|murine thymoma viral (v-akt) oncogene homolog 1|protein kinase B alpha|thymoma viral proto-oncogene 1|v-akt murine thymoma viral oncogene homolog 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Akt1, check out the Akt1 Infographic

Akt1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Akt1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1085 has been cited in 16 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A small interfering RNA targeting osteopontin as gastric cancer therapeutics

Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells

Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia

Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model

Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways

Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways

Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice

Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway

Li R, Cui K, Liu K, Li H, Zhang Y, Liu X, Chen R, Li M, Wang T, Wang S, Liu J, Rao K. Sci Rep. 2017 Oct 18;7(1):13464. doi: 10.1038/s41598-017-12907-1. Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS a...

Fanglin Ma, Zhe Zhou, Na Li, Liming Zheng, Chongyang Wu, Bowen Niu, Furong Tang, Xin He, Guangpeng Li & Jinlian Hua Scientific Reports 6, Article number: 38805 (2016) doi:10.1038/srep38805 Lin28a promotes self-renewal and proliferation of dairy...

Wang Q, Sun X, Li X, Dong X, Li P, Zhao L. Mol Med Rep. 2015 Jan;11(1):151-8. Doi: 10.3892/Mmr.2014.2762. Epub 2014 Oct 23. Resveratrol Attenuates Intermittent Hypoxia-Induced Insulin Resistance In Rats: Involvement Of Sirtuin 1 And The Phosphatid...

Wan J, Che Y, Kang N, Wu W. Mol Med Rep. 2015 Jul;12(1):83-92. Doi: 10.3892/Mmr.2015.3368. Epub 2015 Feb 17. Socs3 Blocks Hif-1?? Expression To Inhibit Proliferation And Angiogenesis Of Human Small Cell Lung Cancer By Downregulating Activation Of ...

Qin Q, Niu J, Wang Z, Xu W, Qiao Z, Gu Y. Cardiovasc Diabetol. 2013 Feb 26;12:37. Doi: 10.1186/1475-2840-12-37. Heparanase Induced By Advanced Glycation End Products (Ages) Promotes Macrophage Migration Involving Rage And Pi3K/Akt Pathway.

Wang G, Li W, Lu X, Bao P, Zhao X. J Diabetes Complications. 2012 Jul-Aug;26(4):259-65. Doi: 10.1016/J.Jdiacomp.2012.04.007. Epub 2012 May 23. Luteolin Ameliorates Cardiac Failure In Type I Diabetic Cardiomyopathy.

Wang Gg, Li W, Lu Xh, Zhao X, Xu L. Croat Med J. 2013 Apr;54(2):171-9. Taurine Attenuates Oxidative Stress And Alleviates Cardiac Failure In Type I Diabetic Rats.

Tang Y, Zhang X, Qi F, Chen M, Li Y, Liu L, He W, Li Z, Zu X. Exp Ther Med. 2015 May;9(5):1851-1856. Epub 2015 Feb 25. Afatinib Inhibits Proliferation And Invasion And Promotes Apoptosis Of The T24 Bladder Cancer Cell Line.

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16 Customer Q&As for Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)

Question

My lab would like using your anti-PKB Alpha antibody (Monoclonal, PKB-175) for lipopolysaccharide-mediated signaling pathway studies. Has this antibody been tested with western blotting on rat ovary tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

We appreciate your inquiry. This MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) is tested on rat ovary tissue, tissue lysate, a549 whole cell lysate, hepa whole cell lysate. It is guaranteed to work for WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-05-01

Question

I was wanting to use your anti-PKB Alpha antibody (Monoclonal, PKB-175) for WB for chicken muscle ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for chicken muscle ovary identification?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

You can see on the product datasheet, MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) has been validated for WB on chicken, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in chicken muscle ovary in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-01

Question

Our team were happy with the WB result of your anti-PKB Alpha antibody (Monoclonal, PKB-175). However we have been able to see positive staining in muscle ovary cytoplasm. using this antibody. Is that expected? Could you tell me where is AKT1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

From literature, muscle ovary does express AKT1. Generally AKT1 expresses in cytoplasm. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-04-24

Question

We are interested in to test anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 on chicken muscle ovary for research purposes, then I may be interested in using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

The products we sell, including anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-27

Question

Will MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Miller

Verified customer

Asked: 2019-10-31

Answer

As indicated on the product datasheet, MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-31

Question

I have attached the WB image, lot number and protocol we used for muscle ovary using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-07

Question

We have been able to see staining in chicken foreskin. Are there any suggestions? Is anti-PKB Alpha antibody (Monoclonal, PKB-175) supposed to stain foreskin positively?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

From what I have seen in literature foreskin does express AKT1. From what I have seen in Uniprot.org, AKT1 is expressed in left adrenal gland, adrenal gland, muscle ovary, foreskin, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have AKT1 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Foreskin, Pubmed ID: 1718748
Liver, Pubmed ID: 24275569
Muscle, and Ovary, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-05-22

Question

Do you have a BSA free version of anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 available?

Verified Customer

Verified customer

Asked: 2019-04-04

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-04

Question

Is this MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) reactive to the isotypes of AKT1?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

The immunogen of MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175) is Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-01-22

Question

Will anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 work for WB with muscle ovary?

A. Krishna

Verified customer

Asked: 2018-04-11

Answer

According to the expression profile of muscle ovary, AKT1 is highly expressed in muscle ovary. So, it is likely that anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 will work for WB with muscle ovary.

Boster Scientific Support

Answered: 2018-04-11

Question

Is a blocking peptide available for product anti-PKB Alpha antibody (Monoclonal, PKB-175) (MA1085)?

C. Thomas

Verified customer

Asked: 2017-10-12

Answer

We do provide the blocking peptide for product anti-PKB Alpha antibody (Monoclonal, PKB-175) (MA1085). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-10-12

Question

We are currently using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?

B. Bhatt

Verified customer

Asked: 2016-09-15

Answer

The anti-PKB Alpha antibody (Monoclonal, PKB-175) (MA1085) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-15

Question

I see that the anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 works with WB, what is the protocol used to produce the result images on the product page?

R. Huang

Verified customer

Asked: 2016-06-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-06-23

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle ovary using anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085. Let me know if you need anything else.

R. Brown

Verified customer

Asked: 2015-07-28

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-07-28

Question

I have a question about product MA1085, anti-PKB Alpha antibody (Monoclonal, PKB-175). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Kulkarni

Verified customer

Asked: 2013-12-12

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1085 anti-PKB Alpha antibody (Monoclonal, PKB-175), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-12-12

Question

Does anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 work on canine WB with cervix carcinoma erythroleukemia?

M. Rodriguez

Verified customer

Asked: 2013-01-02

Answer

Our lab technicians have not validated anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 on canine. You can run a BLAST between canine and the immunogen sequence of anti-PKB Alpha antibody (Monoclonal, PKB-175) MA1085 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-01-02

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MA1085
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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