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Product Info Summary
|Reactive Species:||Chicken, Human, Mouse, Rat|
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Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175)
Boster Bio Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) catalog # MA1085. Tested in WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKB Alpha Akt1 Antibody (Monoclonal, PKB-175) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1085)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Synthetic peptide corresponding to amino acids 461-477 of human PKB alpha/Akt1, conjugated to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1085 is reactive to Akt1 in Chicken, Human, Mouse, Rat
MA1085 is guaranteed for WB Boster Guarantee
Background of Akt1
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1 (ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, mouse, rat, chicken
Validation Images & Assay Conditions
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Anti-PKB alpha antibody, MA1085, Western blotting
All lanes: Anti PKB alpha (MA1085) at 0.5ug/ml
Lane 1: Rat Ovary Tissue Lysate at 50ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 56KD
Observed bind size: 56KD
Gene/Protein Information For Akt1 (Source: Uniprot.org, NCBI)
RAC-alpha serine/threonine-protein kinase
protein kinase superfamily
AKT serine/threonine kinase 1; AKT; Akt1; AKT1m; CWS6; EC 2.7.11; EC 126.96.36.199; PKB alpha; PKB; PKBMGC99656; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; rac protein kinase alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-alpha; RAC-PK-alpha; RACPKB-ALPHA; Serine-Threonine Protein Kinase ; v-akt murine thymoma viral oncogene homolog 1; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1 Akt1|Akt|AKT serine/threonine kinase 1|RAC-alpha serine/threonine-protein kinase|AKT1 kinase|PKB alpha|RAC protein kinase alpha RAC-PK alpha|RAC-PK-alpha|murine thymoma viral (v-akt) oncogene homolog 1|protein kinase B alpha|thymoma viral proto-oncogene 1|v-akt murine thymoma viral oncogene homolog 1*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Akt1, check out the Akt1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Akt1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
MA1085 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A small interfering RNA targeting osteopontin as gastric cancer therapeutics
Short hairpin RNA targeting AKT1 and PI3K/p85 suppresses the proliferation and self-renewal of lung cancer stem cells
Protective Effects of Sodium (?)-5-Bromo-2-(%u03B1-Hydroxypentyl) Benzoate in a Rodent Model of Global Cerebral Ischemia
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model
Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways
Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice
Progesterone alleviates hypoxic-ischemic brain injury via the Akt/GSK-3? signaling pathway
Li R, Cui K, Liu K, Li H, Zhang Y, Liu X, Chen R, Li M, Wang T, Wang S, Liu J, Rao K. Sci Rep. 2017 Oct 18;7(1):13464. doi: 10.1038/s41598-017-12907-1. Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS a...
Fanglin Ma, Zhe Zhou, Na Li, Liming Zheng, Chongyang Wu, Bowen Niu, Furong Tang, Xin He, Guangpeng Li & Jinlian Hua Scientific Reports 6, Article number: 38805 (2016) doi:10.1038/srep38805 Lin28a promotes self-renewal and proliferation of dairy...
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