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Product Info Summary
| SKU: | M01718-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PLAP Rabbit Monoclonal Antibody, Clone#RM317
SKU/Catalog Number
M01718-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-PLAP Rabbit Monoclonal Antibody, Clone#RM317 (Catalog # M01718-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PLAP Rabbit Monoclonal Antibody, Clone#RM317 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01718-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM317
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human PLAP (Placental alkaline phosphatase)
Cross-reactivity
This antibody reacts to human PLAP (Placental alkaline phosphatase).
Reactive Species
M01718-1 is reactive to ALPP in Human
Calculated molecular weight
58.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01718-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of A431 cells lysate using anti-PLAP rabbit monoclonal antibody (Clone RM317) at a 1:2000 dilution.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human placenta tissue section using anti-PLAP rabbit monoclonal antibody (Clone RM317) at a 1:1000 dilution.
Specific Publications For Anti-PLAP Rabbit Monoclonal Antibody, Clone#RM317 (M01718-1)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-PLAP Rabbit Monoclonal Antibody, Clone#RM317
Question
We have been able to see staining in human cervix placenta. Any tips? Is anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 supposed to stain cervix placenta positively?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
From literature cervix placenta does express ALPP. From Uniprot.org, ALPP is expressed in placenta, cervix placenta, cervix carcinoma, among other tissues. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-02
Question
We purchased anti-PLAP Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
I looked at the website and datasheets of our anti-PLAP Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-03-09
Question
Will M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
It shows on the product datasheet, M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-09
Question
I see that the anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-31
Question
I was wanting to use your anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
As indicated on the product datasheet, M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-11
Question
We are currently using anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-08
Answer
The anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 (M01718-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-08
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-07
Question
Is this M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 reactive to the isotypes of ALPP?
Verified Customer
Verified customer
Asked: 2018-09-13
Answer
The immunogen of M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 is A peptide corresponding to the N-terminus of human PLAP (Placental alkaline phosphatase). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-13
Question
We were content with the WB result of your anti-PLAP Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-05-31
Answer
According to literature, cervix placenta does express ALPP. Generally ALPP expresses in cell membrane. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-05-31
Question
Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1. Please let me know if you require anything else.
J. Jackson
Verified customer
Asked: 2018-05-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-28
Question
Is there a BSA free version of anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 available?
J. Patel
Verified customer
Asked: 2018-01-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-10
Question
I am interested in using your anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 for intra-golgi traffic studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
Thanks for your inquiry. This M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 is validated on a431 cells, human placenta tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-11-29
Question
We want to test anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 on human cervix carcinoma for research purposes, then I may be interested in using anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
The products we sell, including anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-24
Question
Is a blocking peptide available for product anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 (M01718-1)?
Verified Customer
Verified customer
Asked: 2017-08-29
Answer
We do provide the blocking peptide for product anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 (M01718-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-08-29
Question
Will anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 work for IHC with cervix carcinoma?
G. Yang
Verified customer
Asked: 2014-08-06
Answer
According to the expression profile of cervix carcinoma, ALPP is highly expressed in cervix carcinoma. So, it is likely that anti-PLAP Rabbit Monoclonal antibody, Clone#RM317 M01718-1 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2014-08-06
Question
Can you help my question with product M01718-1, anti-PLAP Rabbit Monoclonal antibody, Clone#RM317. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Krishna
Verified customer
Asked: 2013-02-14
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01718-1 anti-PLAP Rabbit Monoclonal antibody, Clone#RM317, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-02-14
