Anti-PNK PNKP Antibody

Product Name

Anti-PNK PNKP Antibody

View all PNKP Antibodies

SKU/Catalog Number

A03566

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-PNK PNKP Antibody (Catalog # A03566). Tested in ELISA, WB applications. This antibody reacts with Human, Chimpanzee.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-PNK PNKP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03566)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PNK protein.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03566 is reactive to PNKP in Human, Chimpanzee

Observed Molecular Weight

42 kDa

Calculated molecular weight

57.1 kDa

Background of PNKP

Mammalian PNK (also known as PNKP, Polynucleotide kinase 3'-phosphatase, DNA 5'-kinase/3'-phosphatase and Polynucleotide kinase) catalyzes the 5’ phosphorylation of nucleic acids and can have associated 3’ phosphatase activity, predictive of an important function in DNA repair following ionizing radiation or oxidative damage. PNK is a nuclear protein that is expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03566 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:3,000 - 1:12,000
WB: 1:300 - 1:1,500
This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 57 kDa in size corresponding to PNK by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information PNKP

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