Anti-PPAR gamma/PPARG Antibody Picoband®

Western blot analysis of PPAR Gamma/PPARG using anti-PPAR Gamma/PPARG antibody (A00449-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human SK-OV-3 whole cell lysates,
Lane 4: human MDA-MB-453 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR Gamma/PPARG antigen affinity purified polyclonal antibody (Catalog # A00449-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR Gamma/PPARG at approximately 65 kDa. The expected band size for PPAR Gamma/PPARG is at 65 kDa.

ANXA1 mediated PPARγ-CEBPα pathway to regulate osteoclast differentiation (A) The mRNA level of PPARγ in RAW264.7 cultured with siANXA1-EVs. (B) The mRNA level of CEBPα in RAW264.7 cultured with siANXA1-EVs. (C) The protein level of PPARγ and CEBPα in RAW264.7 cultured with siANXA1-EVs. (D) Quantitative analysis of PPARγ protein expression. (E) Quantitative analysis of CEBPα protein expression. (F) Schematic illustration of PPARγ inhibited RAW264.7 and DFSC-EVs co-culture system. (G) Representative images of TRAP staining. Scale bar = 200 μm. (H) Quantitative analysis of TRAP-positive area. (I) PPARγ inhibited RAW264.7 construction. (J) The mRNA level of CEBPα in PPARγ inhibited RAW264.7. (K) The protein level of PPARγ and CEBPα in PPARγ inhibited RAW264.7. (L) Quantitative analysis of PPARγ protein expression. (M) Quantitative analysis of CEBPα protein expression. (N) The mRNA level of ACP5 , CTSK and CFOS in PPARγ inhibited RAW264.7. (O) The protein level of ACP5, CTSK and CFOS in PPARγ inhibited RAW264.7. (P) Western blotting quantification. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. n = 3.
Index in PubMed under a CC BY license. PMID: 39834384

DFSCs-EVs/ANXA1 regulating tooth eruption by affecting osteoclast differentiation. (A) Representative micro-CT images of detecting tooth eruption distance. (B) Analysis of tooth eruption distance based on micro-CT. (C) Representative H&E staining images of the first mandibular molar area. (D) Analysis of tooth eruption distance based on H&E staining. (E) Representative images of TRAP staining. (F) Quantitative analysis of TRAP-positive area. (G) Representative immunohistochemistry staining images of PPARγ expression in the first mandibular molar area. (H) Quantitative analysis of PPARγ expression in the first mandibular molar area. (I) Representative immunohistochemistry staining images of CEBPα expression in the first mandibular molar area. (J) Quantitative analysis of CEBPα expression in the first mandibular molar area. ns, not significant. Scale bar = 1 mm ** p < 0. 01. n = 3.
Index in PubMed under a CC BY license. PMID: 39834384

IF analysis of PPAR gamma/PPARG using anti-PPAR gamma/PPARG antibody (A00449-3).
PPAR gamma/PPARGNOX4 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPAR gamma/PPARG Antibody (A00449-3) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Flow Cytometry analysis of A431 cells using anti-PPAR Gamma/PPARG antibody (A00449-3).
Overlay histogram showing A431 cells stained with A00449-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPAR Gamma/PPARG Antibody (A00449-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.