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Product Info Summary
| SKU: | M11798-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-PPWD1 Mouse Monoclonal Antibody [Clone ID: OTI1B8]
SKU/Catalog Number
M11798-1
Size
100 µl
Description
Boster Bio PPWD1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8). Catalog# M11798-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-PPWD1 Mouse Monoclonal Antibody [Clone ID: OTI1B8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M11798-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1B8
Isotype
IgG1
Immunogen
Full length human recombinant protein of human PPWD1 (NP_056157) produced in HEK293T cell.
Reactive Species
M11798-1 is reactive to PPWD1 in Human, Mouse, Rat
Calculated molecular weight
73.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M11798-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:200~500
IF 1:100
Flow Cytometry 1:100
IHC: 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPWD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPWD1.
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PPWD1 mouse monoclonal antibody. (M11798-1)
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HEK293T cells transfected with either PPWD1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPWD1 antibody (M11798-1)
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Anti-PPWD1 mouse monoclonal antibody (M11798-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPWD1.
Specific Publications For Anti-PPWD1 Mouse Monoclonal Antibody [Clone ID: OTI1B8] (M11798-1)
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