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TIMM17A antibody (A12168-1) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of A431 cells using anti-TIMM17A antibody (A12168-1).
Overlay histogram showing A431 cells stained with A12168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMM17A Antibody (A12168-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing A431 cells stained with A12168-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TIMM17A Antibody (A12168-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Designing a Western blot or IHC for TIMM17A? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-TIMM17A Antibody Picoband®
TIMM17A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
TIMM17A antibody was positively detected by WB in:
rat heart tissue, rat lung tissue, rat liver tissue, rat brain tissue, mouse heart tissue, mouse lung tissue, mouse liver tissue, mouse brain tissue, mouse Neuro-2a whole cell,, human placenta tissue, human K562 whole cell, human T-47D whole cell, human Caco-2 whole cell, human Hela whole cell, human THP-1 whole cell, human A431 whole cell, human A549 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TIMM17A antibody was positively detected by IHC in:
human liver cancer tissue, human lung cancer tissue, human mammary cancer tissue, rat lung tissue, mouse lung tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TIMM17A antibody was positively detected by ICC/IF in:
HELA cell, A549 cell, A549 cell
TIMM17A antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Human
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-TIMM17A Antibody Picoband®
View all TIMM17A Antibodies
View all TIMM17A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TIMM17A, which shares 88.2% and 82.4% amino acid (aa) sequence identity with mouse and rat TIMM17A, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Observed Molecular Weight
18 kDa
Calculated Molecular Weight
18.0 kDa
Background of TIMM17A
Mitochondrial import inner membrane translocase subunit Tim17-A is an enzyme that in humans is encoded by the TIMM17A gene. It is mapped to 1q32.1. TIM17 is a conserved suppressor of mtDNA instability, and overexpression of TIMM17A can prevent mtDNA loss in a human cellular model of mitochondrial disease.
Cite This Product
Anti-TIMM17A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12168-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
88%
Moderate Homology
Rat
82%
Moderate Homology
Zebrafish
82%
Moderate Homology
Cow
71%
Moderate Homology
Drosophila
59%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TIMM17A antibody
Immunohistochemistry (IHC) protocol for TIMM17A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TIMM17A antibody
Flow Cytometry (FC) protocol for TIMM17A antibody
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Product Promise
We promise Anti-TIMM17A Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A12168-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026