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Product Info Summary
| SKU: | M00290 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Prealbumin TTR Rabbit Monoclonal Antibody
SKU/Catalog Number
M00290
BM4944 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Prealbumin TTR Rabbit Monoclonal Antibody catalog # M00290. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prealbumin TTR Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00290)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOFI-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Prealbumin
Reactive Species
M00290 is reactive to TTR in Human
Observed Molecular Weight
16 kDa
Calculated molecular weight
15.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00290 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
IP 1:20
Positive Control
WB: human fetal kidney
Validation Images & Assay Conditions
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Western blot analysis of Prealbumin gamma expression in human fetal kidney lysate.
Specific Publications For Anti-Prealbumin TTR Rabbit Monoclonal Antibody (M00290)
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5 Customer Q&As for Anti-Prealbumin TTR Rabbit Monoclonal Antibody
Question
We have tried in the past anti-Prealbumin Rabbit Monoclonal antibody for WB on retina in the past. I am using human, and We want to use the antibody for IP next. I would like examining retina as well as platelet in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
I looked at the website and datasheets of our anti-Prealbumin Rabbit Monoclonal antibody and it appears that M00290 has been validated on human in both WB and IP. Thus M00290 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-25
Question
We are currently using anti-Prealbumin Rabbit Monoclonal antibody M00290 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The anti-Prealbumin Rabbit Monoclonal antibody (M00290) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-13
Question
Our team were satisfied with the WB result of your anti-Prealbumin Rabbit Monoclonal antibody. However we have been able to see positive staining in retina secreted. cytoplasm. using this antibody. Is that expected? Could you tell me where is TTR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
From literature, retina does express TTR. Generally TTR expresses in secreted. cytoplasm. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2020-01-13
Question
What is the epitope for M00290?
Verified customer
Asked: 2019-11-27
Answer
The immunogen sequence for the Anti-Prealbumin TTR Rabbit Monoclonal Antibody (M00290) is GPTGTGESKC.
Boster Scientific Support
Answered: 2019-12-03
Question
We have seen staining in human cajal-retzius cell fetal brain cortex. Any tips? Is anti-Prealbumin Rabbit Monoclonal antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
S. Singh
Verified customer
Asked: 2017-06-01
Answer
Based on literature cajal-retzius cell fetal brain cortex does express TTR. Based on Uniprot.org, TTR is expressed in pigmented layer of retina, liver, retina, corpus callosum, platelet, brain, cajal-retzius cell fetal brain cortex, plasma, cerebrospinal fluid, among other tissues. Regarding which tissues have TTR expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1979335
Cerebrospinal fluid, Pubmed ID: 18221012
Corpus callosum, Pubmed ID: 14702039
Liver, Pubmed ID: 1666289, 2015890, 3022108, 6093805, 15489334, 24275569
Plasma, Pubmed ID: 14760718, 16335952
Platelet, Pubmed ID: 12665801
Retina, Pubmed ID: 10328977
Boster Scientific Support
Answered: 2017-06-01
