Anti-PRKAR2A Purified Monoclonal Antibody

Product Name

Anti-PRKAR2A Purified Monoclonal Antibody

View all PRKAR2A Antibodies

SKU/Catalog Number

M03911

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PRKAR2A Purified Monoclonal Antibody (Catalog# M03911). Tested in ICC, WB application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-PRKAR2A Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03911)

Host

Mouse

Contents

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

Hs-36

Isotype

Mouse IgM

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.

Cross-reactivity

This antibody does not cross-react with Thy-1.1 alloantigen.

Reactive Species

M03911 is reactive to PRKAR2A in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

45.5 kDa

Background of PRKAR2A

PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03911 is guaranteed for ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry: 10 μg/ml; membrane permeabilization (acetone) is essential.

Validation Images & Assay Conditions

See full target information PRKAR2A

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