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Product Info Summary
| SKU: | A02171 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Probetacellulin BTC Antibody
SKU/Catalog Number
A02171
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Probetacellulin BTC Antibody catalog # A02171. Tested in WB, ELISA, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Probetacellulin BTC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02171)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human BTC. AA range:21-70
Reactive Species
A02171 is reactive to BTC in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
19.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02171 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
ELISA 1:2000-20000
Validation Images & Assay Conditions
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Western Blot analysis of KB cells using Probetacellulin Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western Blot analysis of KB cells using Probetacellulin Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-Probetacellulin BTC Antibody (A02171)
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7 Customer Q&As for Anti-Probetacellulin BTC Antibody
Question
I was wanting to use your anti-Probetacellulin antibody for WB for human lower esophagus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lower esophagus identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
As indicated on the product datasheet, A02171 anti-Probetacellulin antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lower esophagus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
I see that the anti-Probetacellulin antibody A02171 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-04
Question
Does anti-Probetacellulin antibody A02171 work on horse WB with ovary?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
Our lab technicians have not validated anti-Probetacellulin antibody A02171 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Probetacellulin antibody A02171 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-25
Question
Is this A02171 anti-Probetacellulin antibody reactive to the isotypes of BTC?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The immunogen of A02171 anti-Probetacellulin antibody is Synthesized peptide derived from the Internal region of human Probetacellulin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-02
Question
Do you have a BSA free version of anti-Probetacellulin antibody A02171 available?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Probetacellulin antibody A02171 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-03
Question
Would anti-Probetacellulin antibody A02171 work for WB with lower esophagus?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
According to the expression profile of lower esophagus, BTC is highly expressed in lower esophagus. So, it is likely that anti-Probetacellulin antibody A02171 will work for WB with lower esophagus.
Boster Scientific Support
Answered: 2019-07-02
Question
I have attached the WB image, lot number and protocol we used for lower esophagus using anti-Probetacellulin antibody A02171. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-21
