Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody
Rabbit IgG polyclonal antibody for Prostaglandin E2 receptor EP1 subtype(PTGER1) detection. Tested with WB in Human.
|Product Name||Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody
See all PTGER1 primary antibodies, ELISA kits and proteins
|Storage & Handling||For proper storage of Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for EP1/PTGER1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. EP1/PTGER1 information: Molecular Weight: 41801 MW; Subcellular Localization: Cell membrane; Multi-pass membrane protein; Tissue Specificity: Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart.|
|Cite This Product||Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1583)|
|Specificity||Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody (PA1583) reacts with Human PTGER1, in native form and recombinant. Superfamily members of PTGER1 are not reactive to PA1583.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Prostaglandin E Receptor EP1(387-404aa AWEASSLRSSRHSGLSHF).|
Our Boster Quality Guarantee for Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Western blot, 0.1-0.5μg/ml, Human
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Prostaglandin E Receptor EP1/PTGER1 Antibody (PA1583).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Anti-Prostaglandin E Receptor EP1 antibody, PA1583, Western blotting
All lanes: Anti Prostaglandin E Receptor EP1 (PA1583) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: MM453 Whole Cell Lysate at 40ug
Predicted bind size: 42KD
Observed bind size: 42KD
Protein Target Info (Source: Uniprot.org)
|Protein Name||Prostaglandin E2 receptor EP1 subtype|
|Tissue Specificity||Abundant in kidney. Lower level expression in lung, skeletal muscle and spleen, lowest expression in testis and not detected in liver brain and heart.|
|Alternative Names||Prostaglandin E2 receptor EP1 subtype;PGE receptor EP1 subtype;PGE2 receptor EP1 subtype;Prostanoid EP1 receptor;PTGER1;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein.|
|Molecular Weight||41801 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(q) proteins which activate a phosphatidylinositol-calcium second messenger system. May play a role as an important modulator of renal function. Implicated the smooth muscle contractile response to PGE2 in various tissues.|
*You can search these to find other products in these research areas.
|Background||PTGER1, Prostaglandin E Receptor 1, comprises 402 amino acids with a predicted molecular mass of 41, 858 and has the 7 predicted transmembrane-spanning domains common to all G protein-coupled receptors. The PTGER1 gene is mapped to chromosome 19p13.1. The protein encoded by this gene is a member of the G protein-coupled receptor family. This protein is one of four receptors identified for prostaglandin E2(PGE2). Through a phosphatidylinositol-calcium second messenger system, Gq proteins mediate this receptor's activity. Knockout studies in mice suggested a role of this receptor in mediating algesia and in regulation of blood pressure. Studies in mice also suggested that this gene may mediate adrenocorticotropic hormone response to bacterial endotoxin.|
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.