Product Info Summary
SKU: | M01512 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC-P, IHC-F |
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Product info
Product Name
Anti-Hu CD200 Purified Monoclonal Antibody
SKU/Catalog Number
M01512
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD200 Purified Monoclonal Antibody (Catalog# M01512). Tested in Flow Cytometry, IHC-P, IHC-F application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD200 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01512)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
OX-104
Isotype
Mouse IgG1 kappa
Immunogen
Recombinant human CD200. The mouse monoclonal antibody OX-104 recognizes an extracellular epitope of CD200, a type-1 glycoprotein of the immunoglobulin superfamily, which is expressed in neurons, B and T cell subsets, keratinocytes, follicular dendritic cells, and ovarian cells.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01512 is reactive to CD200 in Human
Applications
M01512 is guaranteed for Flow Cytometry, IHC-P, IHC-F Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
31.264kDa
Background of CD200/OX2
CD200 (also known as OX2 or MRC) is a type-1 membrane glycoprotein, which contains two extracellular immunoglobulin domains, transmembrane domain and cytoplasmic domain. It is expressed by neuronal cells, B and T cell subsets, follicular dendritic cells, keratinocytes, and ovarian cells. The interaction between CD200 and its receptor CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TNF-alpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. It seems CD200 is also involved in maternal tolerance and its decreased expression in hair follicle correlates with follicular miniaturization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry validation of CD200 using Anti-Hu CD200 Purified Monoclonal Antibody (M01512).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD200 (OX-104) purified antibody (concentration in sample 4 μg/ml) GAM APC.Click image to see more details
Figure 2. Flow Cytometry validation of CD200 using Anti-Hu CD200 Purified Monoclonal Antibody (M01512).
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human CD200 (OX-104) purified antibody (concentration in sample 4 μg/ml) GAM APC.Click image to see more details
Figure 3. Flow Cytometry validation of CD200 using Anti-Hu CD200 Purified Monoclonal Antibody (M01512).
Separation of human CD200 positive CD3 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD200 (OX-104) purified antibody (concentration in sample 4 μg/ml) GAM APC.Protein Target Info & Infographic
Gene/Protein Information For CD200 (Source: Uniprot.org, NCBI)
Gene Name
CD200
Full Name
OX-2 membrane glycoprotein
Weight
31.264kDa
Alternative Names
antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2 CD200 MOX1, MOX2, MRC, OX-2 CD200 molecule OX-2 membrane glycoprotein|CD200 antigen|antigen identified by monoclonal antibody MRC OX-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD200, check out the CD200 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD200: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD200 Purified Monoclonal Antibody (M01512)
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No publications found for M01512
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