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PTBP1 antibody (M01798-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of K562 cells using anti- PTBP1 antibody (M01798-1).
Overlay histogram showing K562 cells stained with M01798-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PTBP1 Antibody (M01798-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing K562 cells stained with M01798-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PTBP1 Antibody (M01798-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-PTBP1 Antibody Picoband®(monoclonal, 3H12)
PTBP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PTBP1 antibody was positively detected by WB in:
human Hela whole cell,, human K562 whole cell,, human A549 whole cell,, human HEK293 whole cell,, human Jurkat whole cell,, human Raji whole cell,, rat PC-12 whole cell,, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTBP1 antibody was positively detected by IHC in:
human rectal cancer tissue, mouse intestine tissue, rat intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
PTBP1 antibody was positively detected by ICC/IF in:
Hela cell
PTBP1 antibody was positively detected by IF in:
human tonsil tissue, rat intestine tissue
PTBP1 antibody was positively detected by FC in:
K562 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Immunofluorescence
2μg/ml
Human, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Recommend
Product Information
Product Name
Anti-PTBP1 Antibody Picoband®(monoclonal, 3H12)
View all PTBP1 Antibodies
View all PTBP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
3H12
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human PTBP1 recombinant protein (Position: M1-A504).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
57 kDa
Calculated Molecular Weight
57.2 kDa
Background of PTBP1
Polypyrimidine tract-binding protein 1 is a protein that in humans is encoded by the PTBP1 gene. It is mapped to 19p13.3. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
Cite This Product
Anti-PTBP1 Antibody Picoband®(monoclonal, 3H12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01798-1)
Protocols
Western Blot protocol for PTBP1 antibody
Immunohistochemistry (IHC) protocol for PTBP1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for PTBP1 antibody
Immunofluorescence (IF) protocol for PTBP1 antibody
Flow Cytometry (FC) protocol for PTBP1 antibody
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Product Promise
We promise Anti-PTBP1 Antibody Picoband®(monoclonal, 3H12) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M01798-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026