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- Table of Contents
PTEN antibody (M00006-1) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Immunofluorescent analysis of HeLa cells, using PTEN Antibody .
Designing a Western blot for PTEN? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PTEN Western Blot GuideBundle Save
Anti-PTEN Rabbit Monoclonal Antibody
PTEN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
PTEN antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, rat liver tissue, mouse heart tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
PTEN antibody was positively detected by IHC in:
human kidney tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-PTEN Rabbit Monoclonal Antibody
View all PTEN Antibodies
View all PTEN Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BEI-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PTEN
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
47.2 kDa
Cite This Product
Anti-PTEN Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00006-1)
Protocols
Western Blot protocol for PTEN antibody
Immunohistochemistry (IHC) protocol for PTEN antibody
Publications
Application:
Species:
Feedback loop centered on MAF1 reduces blood–brain barrier damage in sepsis-associated encephalopathy
Published: 2025-01-20
Journal: CELLULAR & MOLECULAR BIOLOGY LETTERS
Application: IHC
Impact Factor: 10.20
Species: Rat
PMID: 39833662
PTEN Deficiency Induced by Extracellular Vesicle miRNAs from Clonorchis sinensis Potentiates Cholangiocarcinoma Development by Inhibiting Ferroptosis
Published: 2024-09-26
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: WB
Impact Factor: 4.90
Species: Human
PMID: 39408679
Oxidized DJ-1 activates the p-IKK/NF-κB/Beclin1 pathway by binding PTEN to induce autophagy and exacerbate myocardial ischemia-reperfusion injury
Published: 2024-03-18
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.20
Species: Rat
PMID: 38508437
Long non-coding RNA LOC366613 alleviates the cerebral ischemic injury via regulating the miR-532-5p/phosphatase and tensin homolog axis
Published: 2021-07-12
Journal: Bioengineered
Application: WB
Impact Factor: 3.27
Species: Mouse
PMID: 34251959
Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation
Published: 2019-10-15
Journal: JOURNAL OF BONE AND MINERAL RESEARCH
Application: WB
Impact Factor: 5.71
Species: Human,Mouse
PMID: 31614022
Participation of tumor suppressors long non-coding RNA MEG3, microRNA-377 and PTEN in glioma cell invasion and migration
Published: 2019-07-23
Journal: PATHOLOGY RESEARCH AND PRACTICE
Application: WB
Impact Factor: 1.79
Species: Human
PMID: 31378455
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons
Published: 2018-09-18
Journal: ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Application: IF
Impact Factor: 1.85
Species: Rat
PMID: 30240910
Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN
Published: 2018-05-21
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 3.33
Species: Human
PMID: 29845233
Effect of compound Guizhu capsule on phosphate and tension homology deleted on chromsome ten and murine double mimute 2 gene expression in lung cancer of mice
Published: 2017-09-18
Journal: Journal of Traditional Chinese Medicine
Application: IHC
Impact Factor: 0.99
Species: Mouse
Expression and clinical significance of EGR-1 and PTEN in the pituitary tumors of elderly patients
Published: 2017-06-13
Journal: Oncology Letters
Application: IHC
Impact Factor: 1.39
Species: Human
PMID: 28789441
Prognostic role of PPAR-γ and PTEN in the renal cell carcinoma
Published: 2015-10-01
Journal: International Journal of Clinical and Experimental Pathology
Application: IHC
Impact Factor: 1.89
Species: Human
PMID: 26722456
Growth suppression of human lung cancer cells and implanted tumors by adenovirus-mediated transfer of the PTEN gene
Published: 2010-04-21
Journal: Journal of Huazhong University of Science and Technology-Medical Sciences
Application: ELISA,IHC
Impact Factor: 0.31
Species: Mouse
PMID: 20407863
Review
Have you used Anti-PTEN Rabbit Monoclonal Antibody?
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1 Review For Anti-PTEN Rabbit Monoclonal Antibody
This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands.
| SKU | M00006-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE. |
| Other Reagents | 5% Non-fat milk |
| Primary Antibody | Anti-PTEN Rabbit Monoclonal Antibody |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent, Imaging system:ChemiDoc MP |
| Results Summary | This antibody is suitable for detecting PTEN protein in mouse hippocampus by Western blot, showing clear, distinct, and highly specific bands. |
Changbin Yuan, LNUTCM
Verified customer
Submitted 2025-11-10
Product Promise
We promise Anti-PTEN Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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M00006-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026