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Product Info Summary
| SKU: | M03998 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody
SKU/Catalog Number
M03998
BM5489 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody catalog # M03998. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03998)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AEAD-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2) . It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) .
Reactive Species
M03998 is reactive to PDHB in Human, Mouse, Rat
Observed Molecular Weight
35 kDa
Calculated molecular weight
39.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03998 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
WB: human K562 whole cell, human HepG2 whole cell, human MCF-7 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Western blot analysis of PDHB using anti-PDHB antibody (M03998).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDHB antigen affinity purified monoclonal antibody (M03998) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PDHB at approximately 35 kDa. The expected band size for PDHB is at 35 kDa.
Specific Publications For Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody (M03998)
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4 Customer Q&As for Anti-Pyruvate Dehydrogenase E1 beta subunit PDHB Monoclonal Antibody
Question
We want to test anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 on human myocardium for research purposes, then I may be interested in using anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
The products we sell, including anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-25
Question
We are currently using anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-12
Answer
The anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody (M03998) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-12
Question
Do you have a BSA free version of anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 available?
N. Jha
Verified customer
Asked: 2016-12-21
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-21
Question
Will anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 work on zebrafish WB with brain?
D. Wu
Verified customer
Asked: 2015-04-23
Answer
Our lab technicians have not tested anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal antibody M03998 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-04-23
