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Product Info Summary
| SKU: | M14567 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Monkey, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-RABL2A Mouse Monoclonal Antibody [Clone ID: OTI4A8]
SKU/Catalog Number
M14567
Size
100 µl
Description
Boster Bio RABL2A mouse monoclonal antibody, clone OTI4A8 (formerly 4A8). Catalog# M14567. Tested in FC, IF, WB. This antibody reacts with Human, Monkey, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-RABL2A Mouse Monoclonal Antibody [Clone ID: OTI4A8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14567)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4A8
Isotype
IgG1
Immunogen
Full length human recombinant protein of human RABL2A (NP_009013) produced in HEK293T cell.
Reactive Species
M14567 is reactive to RABL2A in Human, Monkey, Rat
Observed Molecular Weight
25.9 kDa
Calculated molecular weight
26.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14567 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500~2000
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RABL2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RABL2A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RABL2A monoclonal antibody.
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HEK293T cells transfected with either RABL2A (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RABL2A antibody (M14567)
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Flow cytometric Analysis of Hela cells
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Anti-RABL2A mouse monoclonal antibody (M14567) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RABL2A.
Specific Publications For Anti-RABL2A Mouse Monoclonal Antibody [Clone ID: OTI4A8] (M14567)
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