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GOLGA2 antibody (A05865-1) performs well in ELISA, Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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7. Flow Cytometry analysis of PC-3 cells using anti- GM130 antibody (A05865-1).
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GM130 Antibody (A05865-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing PC-3 cells stained with A05865-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GM130 Antibody (A05865-1,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-GM130/GOLGA2 Antibody Picoband®
GOLGA2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB |
Tested Applications
GOLGA2 antibody was positively detected by WB in:
human HEK293 whole cell, human Jurkat whole cell, human Hela whole cell, human U87 whole cell, human PC-3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GOLGA2 antibody was positively detected by IHC in:
human oesophagus squama cancer tissue, human placenta tissue, human tonsil tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GOLGA2 antibody was positively detected by IHC-F in:
human placenta tissue
GOLGA2 antibody was positively detected by ICC/IF in:
U20S cell
GOLGA2 antibody was positively detected by FC in:
PC-3 cell, U20S cell
GOLGA2 antibody was positively detected by IP in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
2μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Immunoprecipitation
0.5-2μg/ml
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GM130/GOLGA2 Antibody Picoband®
View all GOLGA2 Antibodies
View all GOLGA2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human GM130 recombinant protein (Position: E796-E913).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
130 kDa
Calculated Molecular Weight
113.1 kDa
Background of GOLGA2
Golgin subfamily A member 2 is a protein that in humans is encoded by the GOLGA2 gene. The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Cite This Product
Anti-GM130/GOLGA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05865-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Cow
87%
Moderate Homology
Sheep
86%
Moderate Homology
Rabbit
83%
Moderate Homology
Mouse
82%
Moderate Homology
Rat
82%
Moderate Homology
Dog
82%
Moderate Homology
Zebrafish
73%
Moderate Homology
Drosophila
39%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GOLGA2 antibody
Immunohistochemistry (IHC) protocol for GOLGA2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GOLGA2 antibody
Flow Cytometry (FC) protocol for GOLGA2 antibody
Immunoprecipitation (IP) protocol for GOLGA2 antibody
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Product Promise
We promise Anti-GM130/GOLGA2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IP, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A05865-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026