Anti-Regucalcin/RGN Antibody Picoband™

Regucalcin antibody

Boster Bio Anti-Regucalcin/RGN Antibody Picoband™ catalog # A05900-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A05900-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Regucalcin/RGN Antibody Picoband™

View all Regucalcin Antibodies

SKU/Catalog Number

A05900-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Regucalcin/RGN Antibody Picoband™ catalog # A05900-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Regucalcin/RGN Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05900-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Regucalcin, which shares 87.5% and 84.4% amino acid (aa) sequence identity with mouse and rat Regucalcin, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05900-1 is reactive to RGN in Human, Mouse, Rat

Applications

A05900-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

33 kDa, 43 kDa

Calculated molecular weight

33.253kDa

Background of Regucalcin

Regucalcin is a protein that in humans is encoded by the RGN gene. The protein encoded by this gene is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For RGN (Source: Uniprot.org, NCBI)

Gene Name

RGN

Full Name

Regucalcin

Weight

33.253kDa

Superfamily

SMP-30/CGR1 family

Alternative Names

EC 3.1.1.17; gluconolactonase; GNL; RCsenescence marker protein-30; regucalcin (senescence marker protein-30); Senescence marker protein 30; SMP-30; SMP30regucalcin RGN GNL, HEL-S-41, RC, SMP30 regucalcin regucalcin|epididymis secretory protein Li 41|gluconolactonase|senescence marker protein 30

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on RGN, check out the RGN Infographic

RGN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RGN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Regucalcin/RGN Antibody Picoband™

Question

I see that the anti-Regucalcin/RGN antibody A05900-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-19

Question

Would anti-Regucalcin/RGN antibody A05900-1 work on primate WB with liver?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

Our lab technicians have not validated anti-Regucalcin/RGN antibody A05900-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Regucalcin/RGN antibody A05900-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-08

Question

We are currently using anti-Regucalcin/RGN antibody A05900-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

The anti-Regucalcin/RGN antibody (A05900-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-23

Question

We want to test anti-Regucalcin/RGN antibody A05900-1 on human liver for research purposes, then I may be interested in using anti-Regucalcin/RGN antibody A05900-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-17

Answer

The products we sell, including anti-Regucalcin/RGN antibody A05900-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-17

Order DetailsPrice
A05900-1

100μg

$370
A05900-1-10ug

10μg sample (liquid)

$99
A05900-1-Biotin

100 μg Biotin conjugated

$570
A05900-1-Cy3

100 μg Cy3 conjugated

$570
A05900-1-Dylight488

100 μg Dylight488 conjugated

$570
A05900-1-Dylight550

100 μg Dylight550 conjugated

$570
A05900-1-Dylight594

100 μg Dylight594 conjugated

$570
A05900-1-FITC

100 μg FITC conjugated

$570
A05900-1-HRP

100 μg HRP conjugated

$570
A05900-1-APC

100 μg APC conjugated

$670
A05900-1-PE

100 μg PE conjugated

$670
A05900-1-carrier-free

Carrier Free

$370

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A05900-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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