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Product Info Summary
| SKU: | A13355-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RILPL1 Antibody Picoband®
SKU/Catalog Number
A13355-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RILPL1 Antibody Picoband® catalog # A09697. Tested in WB, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RILPL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13355-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human RILPL1 recombinant protein (Position: E3-K324). Human RILPL1 shares 93.2% and 93.5% amino acid (aa) sequence identity with mouse and rat RILPL1, respectively.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A13355-1 is reactive to RILPL1 in Human, Rat
Observed Molecular Weight
50 kDa
Calculated molecular weight
47.1 kDa
Background of RILPL1
While the function of the Rab interacting lysosomal protein-like 1 (RILPL1) remains unknown, the homologous protein RILP is a small GTPase that controls transport to endocytic compartments, suggesting RILPL1 may have similar functions. However, overexpression of RILP caused enlarged lysosomes that are more centrally located in the cell, while overexpression of RILPL1 had no visible effect on the location or morphology of lysosomes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13355-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human H9C2(2-1) whole cell, human PC-3 whole cell
FCM: 293T cell
Validation Images & Assay Conditions
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Western blot analysis of RILPL1 using anti-RILPL1 antibody (A13355-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human H9C2(2-1) whole cell lysates,
Lane 2: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RILPL1 antigen affinity purified polyclonal antibody (Catalog # A13355-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RILPL1 at approximately 50 kDa. The expected band size for RILPL1 is at 47 kDa.
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Flow Cytometry analysis of 293T cells using anti-RILPL1 antibody (A13355-1).
Overlay histogram showing 293T cells stained with A13355-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RILPL1 Antibody (A13355-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-RILPL1 Antibody Picoband® (A13355-1)
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