|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-RIP2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Receptor-interacting serine/threonine-protein kinase 2(RIPK2) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-RIP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1861)|
|Replacement Item||This antibody may replace the following items: sc-136059|sc-161184|sc-166765|sc-22763|sc-8610|sc-8611 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human RIP2(495-514aa DIQGEEFAKVIVQKLKDNKQ), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Receptor-interacting serine/threonine-protein kinase 2|
|Molecular Weight||61195 MW|
|Protein Function||Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. .|
|Tissue Specificity||Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.|
|Sequence Similarities||Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.|
|Subcellular Localization||Cytoplasm .|
|Alternative Names||Receptor-interacting serine/threonine-protein kinase 2;220.127.116.11;CARD-containing interleukin-1 beta-converting enzyme-associated kinase;CARD-containing IL-1 beta ICE-kinase;RIP-like-interacting CLARP kinase;Receptor-interacting protein 2;RIP-2;Tyrosine-protein kinase RIPK2;18.104.22.168;RIPK2;CARDIAK, RICK, RIP2;UNQ277/PRO314/PRO34092;|
|Research Areas|||cell biology|apoptosis|intracellular|kinases| cell biology|caspases etc|card family| signal transduction|protein phosphorylation|ser / thr kinases|other kinases| cancer|invasion/microenvironment|death receptors & ligands|rip| immunology|innate immunity|tlr signaling| metabolism|types of disease|obesity|cell death|receptors||
Background for Receptor-interacting serine/threonine-protein kinase 2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-RIP2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-RIP2 Antibody Images
Click the images to enlarge.
All lanes: Anti RIP2 (PA1861) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,