Product Info Summary
SKU: | PA1861 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RIP2/RIPK2 Antibody
SKU/Catalog Number
PA1861
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RIP2/RIPK2 Antibody catalog # PA1861. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RIP2/RIPK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1861)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RIP2, different from the related rat and mouse sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1861 is reactive to RIPK2 in Human, Mouse, Rat
Applications
PA1861 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
61 kDa
Calculated molecular weight
61.195kDa
Background of RIPK2
RIPK2 (Receptor-interacting serine/threonine-protein kinase 2), also known as CARD3, CARDIAK, RICK, RIP2, is an enzyme that in humans is encoded by the RIPK2 gene. It has 540-amino acid protein in length. Northern blot analysis revealed that RICK is expressed in various human tissues as 2.5- and 1.8-kb mRNAs that differ due to alternative polyadenylation. RICK is a novel kinase that may regulate apoptosis induced by the FAS receptor pathway. This gene encodes a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal caspase recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappa B and inducer of apoptosis in response to various stimuli, CARDIAK (CARD-containing ICE-associated kinase) specifically interacted with the CARD of ICE/caspase-1, and this interaction correlated with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 subunit.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-RIP2 antibody, PA1861, Western blotting
All lanes: Anti RIP2 (PA1861) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: PANC Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 61KD
Observed bind size: 61KD
Protein Target Info & Infographic
Gene/Protein Information For RIPK2 (Source: Uniprot.org, NCBI)
Gene Name
RIPK2
Full Name
Receptor-interacting serine/threonine-protein kinase 2
Weight
61.195kDa
Superfamily
protein kinase superfamily
Alternative Names
CARD3; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARDIAKCCK; EC 2.7.11; EC 2.7.11.1; GIG30; growth-inhibiting gene 30; receptor interacting protein 2; receptor-interacting protein (RIP)-like interacting caspase-like apoptosisregulatory protein (CLARP) kinase; Receptor-interacting protein 2; receptor-interacting serine/threonine-protein kinase 2; receptor-interacting serine-threonine kinase 2; RICK; RICKGIG30; RIP2; RIP-2; RIP2CARD-carrying kinase; RI RIPK2 CARD3, CARDIAK, CCK, GIG30, RICK, RIP2 receptor interacting serine/threonine kinase 2 receptor-interacting serine/threonine-protein kinase 2|CARD-carrying kinase|CARD-containing IL-1 beta ICE-kinase|CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase|RIP-2|growth-inhibiting gene 30|receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase|receptor-interacting protein 2|tyrosine-protein kinase RIPK2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RIPK2, check out the RIPK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RIPK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RIP2/RIPK2 Antibody (PA1861)
Hello CJ!
No publications found for PA1861
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RIP2/RIPK2 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-RIP2/RIPK2 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-RIP2/RIPK2 Antibody
Question
Will anti-RIP2/RIPK2 antibody PA1861 work for WB with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
According to the expression profile of cervix carcinoma erythroleukemia, RIPK2 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-RIP2/RIPK2 antibody PA1861 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-12-18
Question
I would like to test anti-RIP2/RIPK2 antibody PA1861 on mouse cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-RIP2/RIPK2 antibody PA1861 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
The products we sell, including anti-RIP2/RIPK2 antibody PA1861, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-02
Question
I see that the anti-RIP2/RIPK2 antibody PA1861 works with WB, what is the protocol used to produce the result images on the product page?
F. Taylor
Verified customer
Asked: 2014-04-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-23
Question
We are currently using anti-RIP2/RIPK2 antibody PA1861 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
R. Wu
Verified customer
Asked: 2013-05-22
Answer
The anti-RIP2/RIPK2 antibody (PA1861) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-22